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Effects of Resveratrol on the Apoptosis and Fas/FasL Apoptotic Pathway in Rabbits with Atherosclerosis

Author: ShangXiuLing
Tutor: ZhuPengLi
School: Fujian Medical
Course: Geriatrics
Keywords: Atherosclerosis Apoptosis Resveratrol Fas / FasL apoptotic pathway
CLC: R285.5
Type: Master's thesis
Year: 2008
Downloads: 117
Quote: 1
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Abstract

Objective: To establish a rabbit atherosclerosis (AS) model to observe the impact of resveratrol on apoptosis in rabbit atherosclerotic plaques, and Fas / FasL apoptotic pathway, from the animal level to reveal resveratrol anti artery atherosclerosis mechanism. Methods: 70 mature healthy male New Zealand white rabbits were randomly divided into five groups, a week after the adaptive feeding A blank control group: normal diet normal saline, B, pathological control group: high fat diet for normal saline, C Pathologic low dose Res groups: high fat diet Res (4mg/Kg/d) gavage, D, pathological control medium dose Res group: the fat feed Res (8mg/Kg/d) gavage, E, pathology dose the Res group: high fat diet Res (16mg/Kg/d) by gavage. High fat diet (recipe: 1% cholesterol of 5% lard the eleven 5% egg yolk powder 11 0.5% bile salts 11 88.5% normal diet). Group A ordinary diet high fat diet of group B, C, D, E group high fat diet and at the same time were given low, medium and high dose resveratrol intervention. At the weekend, 12 animals were sacrificed and specimens were taken. Changes in serum lipids blood glucose to observe the morphological changes of aortic pathology, and TUNEL staining detection rate of apoptosis of vascular wall, reverse transcriptase polymerase chain reaction assay FasmRNA, FasLmRNA, Caspase-3mRNA level. , P lt; 0.05 was considered statistically significant differences were analyzed by ANOVA and non-parametric tests using SPSS statistical software. Results: (1) the high fat diet for 12 weeks, elevated pathology group lipid TC, TG, LDL-C, of ??HDL-C compared with normal control group (P lt; 0.01); pathology group, resveratrol The intervention group (the pathological low dose Res group, pathological dose Res group, pathological high doses Res group) can reduce AS rabbit serum TG, LDL-C (P lt; 0.01), with the dose of growth reduction greater (P lt; 0.01); elevated pathological middle dose the Res group and pathological HDL-C, high-dose Res group, the difference was statistically significant (P lt; 0.01); three doses of resveratrol intervention group TC no significant change (P gt; 0.05); blood glucose before and after the experiment was no significant difference (P gt; 0.05). (2) high fat diet for 12 weeks after the successful establishment of the AS model, pathology severe AS pathological changes in the control group, the control group without AS plaque resveratrol intervention group AS lesions significantly reduced plaque area was significantly reduced ( P lt; 0.05), endometrial thickness decreased significantly (P lt; 0.05), and was dose-dependent. (3) pathological control group AS plaque large number of apoptotic cells in the intima of the normal control group occasionally a small number of apoptotic cells (P lt; 0.01); resveratrol intervention group inhibit plaque within cells apoptosis, the the pathological low doses Res group (P lt; 0.05), pathology middle dose the Res group and pathological high dose Res group (P lt; 0.01); resveratrol pairwise comparisons of the treatment group difference significant sex (P lt; 0.01). (4) pathological control group AS plaque FasmRNA high expression, normal control group with low expression (P lt; 0.01); pathology group, resveratrol intervention group (pathology dose Res and pathological high doses of Res The) FasmRNA expression significantly decreased (P lt; 0.01); pathological high-dose the Res group with normal control group, the difference was not significant sex (P gt; 0.05), this is the first time in the country report. (5) pathological control group and pathological low dose Res the group FasLmRNA expression was significantly increased (P gt; 0.05) and normal control group FasLmRNA little expression (P lt; 0.01); resveratrol intervention group (pathological middle dose the Res group and pathological the high dose Res group) FasLmRNA expression was significantly decreased (P lt; 0.01); compare differences between any two of the three doses of resveratrol intervention group was significant (P lt; 0.01). (6) pathological control group and pathological low dose of Res Caspase-3mRNA expression significantly increased (P gt; 0.05), normal controls expression of Caspase-3mRNA small amount of expression (P lt; 0.01); pathology dose Res group and pathological high dose The the Res group of Caspase-3mRNA expression significantly decreased (P lt; 0.01); three doses of resveratrol intervention group expression of Caspase-3mRNA turn reduce pairwise comparisons among the three groups, the difference was statistically significant (P lt; 0.01). (7) Caspase-3mRNA expression and FasmRNA of expression was a significant positive correlation (r = 0.793, P lt; 0.01). Conclusion: Resveratrol inhibits AS plaque apoptosis was significantly inhibited AS lesions progress, this role may improve dyslipidemia, inhibit plaque apoptosis, inhibition of Fas / FasL apoptotic pathway, reducing Caspase 3 gene expression, and the dose-effect relationship.

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