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Objective: Screening Ling Ju seven different extraction methods extract the best hypoglycemic components and detect their security, and to observe the blood sugar of alloxan hyperglycemic mice, oral glucose tolerance, serum insulin levels; visits Ling Ju seven extracts of rat model of type 2 diabetes therapeutic effect and T2DM vascular endothelial injury, initially to investigate the mechanism of its hypoglycemic effect. Methods: (1) male Kunming mice by intraperitoneal injection of alloxan diabetes was experimental mouse model, ig Ling Ju Seven extract best hypoglycemic effect Ling Ju seven extracts screened for preliminary the pharmacological experiments. Seven extract of acute toxicity test to 2.5g/ml Ling Ju 1ml mice administered orally observed 7d, and record the mouse response. (2) to gavage study on blood glucose, oral glucose tolerance, serum insulin levels in alloxan diabetic mice without dose Ling Ju seven extracts. (3) SPF male SD rats with high sugar and fat feeding combined with low-dose streptozotocin (STZ)-induced prepared with type 2 diabetes model, grouping Ling Ju the heptahydrate extract and positive control drugs were given continuous 30d . Harvested after the end of administration and detection of blood glucose (BG), blood lipids (triglycerides TC, total cholesterol and TG), serum insulin (Ins), superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide ( NO), plasma endothelin (ET), taken pancreas HE and immunohistochemical staining observed seven extracts investigated Ling Ju indicators. Results: (1) Ling Ju seven best hypoglycemic group divided into a water extract group mice its maximum tolerated large 125g/kg, equivalent to 278 times the adult daily dose; (2) Ling Ju seven water extraction material each dose could reduce the blood sugar, improve oral glucose tolerance and serum insulin levels, in which the effect of the high dose group. (3) Ling Ju seven extracts may significantly reduce type 2 diabetic rat model of blood sugar, improve its dyslipidemia and hyperinsulinemia, increased antioxidant enzyme activity in the body, reducing lipid peroxidation, protection T2DM vascular endothelial function, promote repair of pancreatic β cells. Conclusion: Ling Ju Seven aqueous extract of hypoglycemic effect, and has a certain amount of efficiency, timeliness relations, and toxicity, oral safety, better prevention and treatment of T2DM vascular endothelial injury of type 2 diabetic rats has a protective effect, hypoglycemic mechanism may enhance the body's ability to scavenge active oxygen free radicals to inhibit free radical damage of pancreatic β-cells, to promote the restoration of islet β-cells.
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