Dissertation > Excellent graduate degree dissertation topics show

Effects of Aqueous Extracts from Lycopsis Orientalis Linn on Skin Ulcer and Its Mechanisms in Mice

Author: SunWenXiao
Tutor: ZhangHaiGang
School: Third Military Medical University
Course: Pharmacology
Keywords: Wolf Lithospermum Surface Ulcers Analgesic Inflammation Cytokines Cyclooxygenase Inhibitory effect
CLC: R285
Type: Master's thesis
Year: 2007
Downloads: 92
Quote: 0
Read: Download Dissertation

Abstract


Skin ulcer is a common refractory disease, the major cause of trauma, local blood circulation disorder, infection, their common feature is wound prolonged unhealed repeated course. With the improvement of people's living standards, the increase of the aging population, the incidence of diabetic ulcers and venous leg ulcers is also on the rise. The ulcers not only from the physical and mental patients with pain, also brought heavy economic burden to the society and the family. Occur in the healing process of the skin ulcer, inflammation throughout play an important role, not only caused damage Ulcers persistent, but also affect the recovery of the ulcer. However, the commonly used anti-inflammatory antimicrobial drugs is not an effective solution ulcer inflammatory process in cytokine expression disorder status, limited efficacy. The wolf Shikonin (Lycopsis orientalis Linn.) A Boraginaceae annual or more annual plants in our country folk used to treat sores has a long history, to good effect. But a long time, at home and abroad is still a lack of pharmacodynamic mechanisms of systems research. To this end, the study on the basis of the successful preparation of the wolf the Lithospermum effectively extract, using a variety of inflammatory pain animal models from multiple levels of its effect and its mechanism of anti-skin ulcers study aims to find novel antibody The table further development and application of the ulcer drug and wolf Lithospermum provide experimental data and theoretical basis. Method: decoction prepared wolf the Lithospermum water extracts (aqueous extracts from lycopsis orientalis linn, AELO). Acetate chemical stimulation produced a skin ulcer model in mice, observed of AELO ulcer healing; spectrophotometric determination of ulcers organization of PGE 2 content; ELISA measurement of serum IL-1β, IL -6, and cytokines such as TNF-α level. By acute peritonitis model in mice, using spectrophotometric detection capillary permeability. Mouse acetic acid writhing test and the hot plate test to determination AELO the pain threshold in mice. Were used Westernblotting and RT-PCR assay the local skin ulcers organization cyclooxygenase -2 (cyclooxygenase-2, COX-2) protein and mRNA expression. Flat punch method and the disk diffusion method, determination of the wolf Lithospermum water extract antibacterial potency and strain its sensitivity test. Results: 1. Wolf Lithospermum 3 days and 5 days after topical application to mouse skin ulcer severity score was significantly improved, especially in the first five days is the most obvious. Topical application of AELO 3 ~ 7 days after ulcer tissue in mice significantly reduced PGE 2 content; compared with the control group, 3, 5 and 7 days, respectively, a decrease of 57.6%, 66.4% and 46.1%, respectively. Mouse hot plate test and writhing test, the the wolf the Lithospermum treatment group (10 ~ 40g/kg) mice pain threshold time was significantly prolonged the writhing reaction times were significantly reduced. A large number of acute peritonitis in mice experiments, the model group mice by intraperitoneal Evans blue extravasation, significantly higher than the normal group; wolf of Lithospermum each dose treatment group in Evans blue extravasation were significantly decreased, especially wolves Lithospermum dose treatment group is the most obvious. Six hours after the mice with acute peritonitis model, IL-1β, IL-6, and TNF-α levels in the serum was significantly increased after application, AELO (10 ~ 40g/kg), IL-1β, IL-6 and TNF-α concentrations were significantly lower (p <0.05), and lower degree AELO dose showed a significant positive correlation. Normal mouse skin tissue almost no COX-2 expression the the model mice surface ulcers Organization of COX-2mRNA in its protein expression were significantly increased, application after AELO treatment, both expression levels were significantly lower. 5 Flat punch Determination the the antibacterial potency paper method liquid sensitivity test, the wolf Shikonin water extract of Escherichia coli, Staphylococcus aureus and Enterococcus faecalis significantly inhibited; inhibit beta-hemolytic streptococcus. Conclusion: 1. Wolf the Lithospermum water extract can significantly promote the healing of skin ulcers in mice. 2 wolf the Lithospermum water extract has significant analgesic effect and capillary permeability, can effectively reduce inflammation, reduce IL-1β, IL-6, and the generation of cytokines such as TNF-α, COX-cut ulcers 2 and its mRNA expression to reduce of PGE 2 generation. 3. Wolf the Lithospermum water extract has good in vitro inhibition of Escherichia coli, Staphylococcus aureus and Enterococcus faecalis.

Related Dissertations

  1. The Effect of Hemoperfustion in Different Time on the Proinflammatory Cytokines and Survival Time of Sepsis Rabbits,R459.7
  2. The Polymorphisms of TLR4 Gene and Function Analysis of C1027A in Suzhong Pig,S828
  3. The Study on Influence of Transcriptional Profiles of Porcine Peripheral Blood Lymphocytes Cultures Following Infection with Porcine Parvovirus,S858.28
  4. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  5. Effects of Aspirin and Curcumin on COX-2, β-attention and PGE2 Expressions in Colon Cancer Cell Lines of HT-29 and SW 480 Cells,R735.35
  6. The Experimental Research of RNAi Technology Inhibiting Respiratory Syncytial Virus,R725.6
  7. Chemical Synthsis, Activity Evaluation and Expression of the Novel Conotoxins from South China Sea,TQ464
  8. Effects of Geniposide and Its Metabolite Genipin on Human Peripheral Blood T Lymphocytes,R758.23
  9. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  10. Investigation of the Immunopathogenesis of Henoch-Schonlein Purpura,R554.6
  11. Effects of Blockade of IL-17 in Already Established Asthma of BALB/c Mice,R562.25
  12. The Function and Mechanism of Endoplasmic Reticulum Stress Pre-treatment on the Improvement of Renal Tolerance Toward Ischemia-reperfusion Injury,R692.5
  13. Preliminary Study to the Xinnaojing Injections in Treatment of Febrile Seizures,R720.597
  14. The Roles of Spinal Cord Glia in Bee Venom-induced Nociception and Inflammation in Concious Rats,R741
  15. Relationship between Expression of COX-2 and Cell Cycle Regulatory Proteins in Patients with Esophageal Squamous Cell Carcinoma,R735.1
  16. LPS activation of complement on endothelial cells and the inhibition of complement on LPS- induced acute lung injury,R563.8
  17. Bronchial asthma serum interleukin -16 Determination and clinical significance,R725.6
  18. Series of rare earth - phenanthroline - indole- B ( d ) acid complexes inhibition,R96
  19. The Effects of Low-arsenic Exposure in Drinking Water on Cardiovascular System and the Function of Dietary Factors,R151
  20. The Preparation of Flurbiprofen-chitosan Hydrogel in Situ and Study on Intrawound Analgesia Effect Afterincisional Administration to Rats,R614
  21. Cord blood cytokine atopic quality family,R722.1

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile