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Preparation and Characterization of Quantum Dots Fluorescence Probes

Author: LiDeNa
Tutor: ChangJin
School: Tianjin University
Course: Materials Science
Keywords: Quantum dots Amphiphilic polymer Phacoemulsification Biological detection
CLC: TH744.1
Type: Master's thesis
Year: 2007
Downloads: 442
Quote: 5
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Abstract


Quantum dots, also known as the inorganic nanocrystals, due to its great future as a fluorescent probe in the biological field biologist who has caused widespread concern. This paper two ways of quantum dot fluorescence probe study: (1) colloidal chemical prepared by oil-soluble CdSe quantum dots with good crystallinity. Discussed during the preparation of reaction time and reaction temperature on the particle size and the luminescent properties of quantum dots prepared. And UV - visible spectrophotometer (UV-Vis), fluorescence spectrometer (PL) and the samples were characterized by transmission electron microscopy (TEM). Found at 250 ℃, with the extension of the reaction time from 4s to 600s, the particle size of CdSe quantum dots gradually increases from 2.85nm to 4.77nm, while absorption and emission spectra red shift. The quantum dots are prepared in the reaction time for 10s reach the maximum fluorescence efficiency. When the reaction time of 30s, and the reaction temperature rose from 210 ℃ to 260 ℃, the particle size of CdSe quantum dots gradually increases from 3.35nm to 4.10nm, absorption and emission spectra are also red-shifted. The preparation of quantum dots is the fluorescence quantum yield decreases with increasing temperature. The analysis revealed that the extension of time or temperature increases have the same impact on the growth of quantum dots, with maximum fluorescence quantum yield when the system prepared CdSe quantum dot emission wavelength of 605.6nm. Continuous ion layer adsorption reaction (SILAR) prepared two different core / shell structure composed of quantum dots, CdSe / CdS / CdS / CdS and CdSe/CdS/Cd0.5Zn0.5S/ZnS type. Found simply by CdS quantum dots prepared by the shell layer fluorescence quantum yield can be increased to 8.4 times the of prokaryotic quantum point added of cadmium zinc alloy prepared core-shell quantum dots can improve its fluorescence quantum yield of 10.6 times, after explaining The CdSe core better passivation protective effect. (2) meet the biological testing requirements in order to obtain water-soluble quantum dot fluorescent probes. Amphiphilic polymer-coated quantum dots microspheres were prepared by ultrasonic emulsification method (O / W). And mercapto acetic acid, and silica modified quantum dots for comparison. The results show that, the phacoemulsification prepared QDs / polymer composite microspheres have better structural stability than thioglycolic acid-modified quantum dots, while the fluorescence properties of silica-coated quantum dots. Prepared in this way the composite microsphere size between 100nm-500nm, by the hydrophobicity of the polymer in the system of the change, the aqueous phase oil Matches ratio, the proportion of the organic phase, the choice of solvent and polymeric substance and quantum dots and other conditions, effective control can be realized on the particle size. Prepared by this method of quantum dots as fluorescent probes in a fluorescence immunoassay can accurately identify the specific antibody, is expected to be further used in biological testing.

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