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The Influence of Space Flight on Germplasm of Glycyrrhiza Uralensis. Fish

Author: LiKeFeng
Tutor: GaoWenYuan
School: Tianjin University
Course: Pharmacognosy
Keywords: Licorice Spaceflight Artificial selection Physiological and biochemical characteristics Genome Active ingredient
CLC: S567.71
Type: Master's thesis
Year: 2007
Downloads: 88
Quote: 2
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Abstract


In this paper, the \artificial selection group (black new WX1) compared to the mutagenic effects of space environment on licorice, and lay the foundation for the quality of screening quality licorice seeds, licorice improve cultivated. The experimental results are as follows: (1) The space environment affect seed germination and radicle growing: in conditions of sufficient water, the satellite equipped group and ground control group of seed germination and radicle growth is no significant difference (P GT; 0.05) , WX1 germination rate and radicle growing significantly higher than the ground control group, a significant difference; growing under drought stress (moderate stress, osmotic pressure-1.0MPa, the same below) WG1 seed germination and radicle to be significantly higher than the WX1 and ground control group (P lt; 0.05), no significant difference between the ground control group and WX1. (2) space environmental impact licorice seed germination process of the soluble protein and enzyme activity metabolism: in conditions of adequate water, the WG1 seed soluble protein, peroxidase, catalase changes and trends and ground control group change is basically the same, and the WX1 soluble protein content and change than the control group and WG1 there are significant differences between; the 3d seed germination under drought stress WG1 seed soluble protein content, peroxidase and catalase in 5d after germination were significantly higher than the ground control group and WX1 significant difference (P lt; 0.05). (3) The space environment can cause the licorice total genome mutagenesis: ISSR analysis of the WG1 licorice seeds genome results show that the 22 pairs of primers produced a total of 132 bands, including polymorphism primer 6 pairs amplified polymorphic bands of 28, 21.2% of the total bands, indicating that the WG1 seed genome has changed compared to the ground control group, while no significant difference between the WX1 genome bands position and brightness control group. (4) space environment affects the synthesis of the active ingredient in licorice root: HPLC analysis of the WG1 root licorice main active ingredient showed that the WG1 licorice the acid and liquiritin the content was significantly higher than the content of the ground control group. Glycyrrhizic acid is 2.19 times the ground control group, glycyrrhizin content is 1.18 times that of the control group, but compared with the WX1, its content is lower than the the licorice root WX1 acid, glycyrrhizin and licorice polysaccharide content.

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CLC: > Agricultural Sciences > Crop > Economic crops > Medicinal crops > Thermophilic drugs > Licorice
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