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The Studies the Effect of Mycophenolate Mofetil on Human Hepatoma Carcinoma Cell HepG-2 Cells

Author: LiuYi
Tutor: MaQingJiu
School: Fourth Military Medical University
Course: Surgery
Keywords: Mycophenolate mofetil Hepatic carcinoma Apoptosis Cell cycle Adhesion
CLC: R735.7
Type: Master's thesis
Year: 2008
Downloads: 43
Quote: 2
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Abstract


Mycophenolate mofetil (MMF; trade name: MMF) is mycophenolic acid (MPA), morpholino ethyl single phosphate inosine dehydrogenase (IMPDH), a purine ribose, amino acids, one-carbon units and CO2 and other simple substance is synthesized purine nucleotide process of one of the key enzymes of the MMF selective effect on the eukaryotic cell of IMPDH blocking DNA synthesis, thus inhibiting the synthesis of purine nucleotide. Clinical mycophenolate mofetil as an immunosuppressive agent has been widely used in the field of organ transplantation. Primary liver cancer is malignancy behind the stomach, esophagus door attainments of one of the common malignancies exchange mortality third place in the rural areas in parts of the United States accounted for second place after gastric our application each year die Jovan 11 liver cancer accounted for 45% of liver cancer deaths worldwide. The emergence of liver transplant brings hope for many patients with liver cancer. Objective: In this study, by mycophenolate mofetil role in liver cancer cells HepG-2 observed mycophenolate mofetil on cell proliferation, apoptosis, adhesion, to explore its possible clinical significance in liver transplantation. Methods: The morphological changes in different time periods role in liver cancer cells, MTT method inhibition rate of tumor cell growth, cell cycle was detected by flow cytometry, Hoechst 33258 staining of apoptotic cells was observed with different concentrations of mycophenolate mofetil cell adhesion assay cell adhesion rate. The incorporation of [3H] - thymidine caused by the determination of cell proliferation. PCR method to detect the expression of adhesion factor of ICAM-1 and VCAM-1. Results: The concentration of 100μg/ml mycophenolate mofetil role in hepatoma cells 72h of liver cancer cells growth inhibition rate (78.8 ± 7.09)%. Hoechst33258 staining results are shown: the nuclear condensation phenomenon 100μg/ml mycophenolate mofetil role in liver cancer cells after 24h after 72 hours of the occurrence of nuclear fragmentation, flow cytometry, cell cycle block in GO/G1, 100μg / ml concentration of mycophenolate mofetil for 72h, G0-G1 phase cell distribution rate of 68.22%. Determination of the incorporation of [3H] - thymidine induced cell proliferation showed that the concentration was 100μg/ml the mycophenolate mofetil role in liver cancer cells, cpm value of 2402.23 ± 142, significantly inhibited the proliferation of hepatoma cells. On PCR results: concentration mycophenolate mofetil concentration of 100μg/ml experimental group / control group ratio were 0.42 ± 0.05,0.51 ± 0.03, ICAM-1, VCAM-1 expression gradually weakened with increasing concentrations of expression . Conclusion: Mycophenolate mofetil inhibits hepatoma cell HepG-2 growth, induction of apoptosis, cell growth arrest in the G1 phase of the cell cycle analysis showed significantly lower S-phase fraction and reduce cell adhesion, inhibition of proliferation of tumor cells, with the time and dose dependence. Prompted mycophenolate mofetil may inhibit hepatocellular carcinoma.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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