Dissertation > Excellent graduate degree dissertation topics show

The Study on Sustained Release of EGF-loaded Microspheres and Its Repair Effect on Diabetic Ulcer

Author: DongXiaoQing
Tutor: ChangJin
School: Tianjin University
Course: Materials Science
Keywords: Microspheres EGF PLGA Double Emulsion Method Cell Culture In Vivo Test
CLC: R587.2
Type: Master's thesis
Year: 2007
Downloads: 144
Quote: 0
Read: Download Dissertation

Abstract


The modified w/o/w extraction–evaporation technique was chosen to prepare epidermal growth factor （EGF） loaded microspheres using poly （lactic-co-glycolic） acid （PLGA）. Bovine serum albumin（BSA） was used as molding drug. The emulsification concentration of inner aqueous phase, the mixture ratio of solvent, the emulsification concentration of outer aqueous phase, pH value, salt concentration was researched for the highest encapsulation efficiency. The microspheres were characterized for morphology by transmission electronic microscopy （TEM）. The particle sizes of microspheres were determined by Particle Size Analyzer. The release performances of EGF loaded PLGA microspheres in vitro were studied, and EGF was measured by enzyme-linked immunosorbant assay （ELISA）. The result showed that microspheres were smooth and spheriacal with a mean particle size 193.5nm, the index of polydispersity was 0.176, and the encapsulation efficiency was 85.6%..There was a linear relationship between the amount of accumulative release Q and time square root t1/2 Q=46.80738+10.09451t1/2（R=0.94341）. The release of EGF from PLGA microspheres can persist for 24h.The proliferation effects of microspheres on fibroblast were evaluated by MTT assay. We found that all of concentration of EGF loaded microspheres can accelerate the proliferation of fibroblast, however, there were some relationships between the concentration of microspheres and the proliferation effects. The result showed that 10μg/L was the optimal concentration for the proliferation. The proliferation effects of 10μg/L EGF loaded microspheres were much better compared with equal EGF. The proliferation effects of different concentration of blank microspheres on fibroblast were investigated. There was no toxicity on fibroblast with blank microspheres concentration range of 0.01μg/L to 100μg/L. All values were treated by statistical analysisThe diabetic ulcer model rats were used for researching the healing effects of EGF loaded microspheres. The change of ulcer areas visual displayed that there was significantly enhanced wound closure with EGF loaded microspheres from the 14th to 21th day compared with the control （EGF solution, the blank microspheres and PBS treatment） groups. Histological examination, the expression of the proliferative cell nuclear antigen（PCNA） and EGF receptor（EGFR）, observation with electron microscope were used to observe the theraputic effects in microscopic view. Firstly, the results showed that the blank microspheres was non-toxic and no inhibited action. Secondly, EGF activity was well maintained by EGF loaded microspheres, which can release EGF slowly and stimulate fibroblast continuously. The healing effects of EGF loaded microspheres were more effective than traditional EGF solution treatment.This work offers evidences for treating the diabetic ulcer using EGF loaded microspheres in clinic.

Related Dissertations

  1. Purification of Human Respiratory Syncytial Virus Fusion Protein with Immunomagnetic Microsphere Technology,R373
  2. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  3. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  4. A Simple and Efficient Method That Uses Low Concentration Fetal Bovine Serum to Culture and Purify Schwann Cells,R329
  5. Bevacizumab -PLGA Microspheres intravitreal injection in rabbit eyes pharmacokinetics and distribution of drugs,R96
  6. Copper oxide porous hollow microspheres Preparation and Characterization,O614.121
  7. For drug ingredients isolated molecularly imprinted polymer microspheres preparation , properties and application of testing,O631.3
  8. Intravitreal injection of bevacizumab PLGA Microspheres Synthesis and identification of,R943
  9. Based magnetic fluid assembled hollow magnetic carbon microspheres and functional complexes,TB383.4
  10. Preparation and Performance of Meloxicam Loaded PLA Sustained-release Microspheres,R943
  11. Carbon nanotubes in the polymer nanofiber surface assembly and cell activity evaluation,R318.08
  12. Preparation of hollow spheres of titanium dioxide and biological activity,TB43
  13. bFGF chitosan microspheres and promote Schwann cell proliferation studies,R651.2
  14. Cloning of Fucal in Monopterus Albus and the Differentiation Expression of SSEA and Fucal in Monopterus Albus Gonad Tissue,S917.4
  15. The Expression of Cortistatin in Psoriasis and the Effect of Exogenous Cortistation on a Proliferation Model of EGF-induced HaCaT Cell,R758.63
  16. The Anti-proliferative Effect of Paclitaxel Polylactic Acid Microsphere on Scarring of Filtering Passageways in Rabbit Eyes after Trabeculectomy,R779.6
  17. Effects of EGF and EGFR on Prognosis of Reflux Esophagitis Patients,R571
  18. The Effects of Hypoxia on the Chondrocytes Differentiation Ability of Human Umbilical Cord-derived Mesenchymal Stem Cells,R329
  19. The Promotive Effect of HGF Gene on the Proliferation of Endothelial Progenitor Cells,R329
  20. Human Umbilical Cord Blood Endothelial Progenitor Cells Separated, Cultured and Identified,R329
  21. Hyaluronic Acid Gel Induction MDSC Differentiation for Chondroid Cells,R329

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetic coma and other complications
© 2012 www.DissertationTopic.Net  Mobile