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The the Tokyo Pterocarya the Pterocarya tonkinesis (Franch. of) the Dode Pterocarya plants Juglandaceae, riverbank quarter rain forest in Xishuangbanna, Yunnan Province, China on behalf of the community types. The plants in some regions of China folk as herbal medicine for the treatment of cancer, currently only a few papers carried a report on its chemical composition and pharmacological studies. Our group in previous work by the screening of anti-tumor activity of thousands of herbal medicine found that ethanol extract of the bark of the Tokyo Pterocarya show strong induced apoptosis and necrosis cytotoxic activity. In order to clarify the anti-tumor activity of the Tokyo Pterocarya ingredient, its chemical composition and its anti-tumor activity. The pre-experimental results indicate that the active site of Tokyo Pterocarya stenoptera its water suspension chloroform and ethyl acetate extracts of the aqueous ethanol extract, for the strong activity of the chloroform extract. Accordingly, Tokyo the Pterocarya stenoptera dried bark (5.0 kg) pulverized, washed with 80% aqueous ethanol at room temperature extraction, recovering ethanol until no alcohol flavor, followed by extraction, with an equal amount of chloroform and ethyl acetate to give activity of the chloroform extract under reduced pressure . The chloroform extract 62 g, using silica gel, polyamide, Sephadex LH-20 such as column chromatography and PTLC, PHPLC separation means, separated from the chloroform extracts of the eight compounds 1 to 8 is obtained. Further use of physicochemical and spectroscopic methods (UV, IR, 1D-NMR, 2D-NMR, MS) the chemical structure of these compounds. The results of estimating the possible structure of compound 1 (1,2,3,4-tetrahydro-1 ,5-dihydroxy-2-isopropyl-naphthalen-8-yl) (1,2-dihydro-1 ,5-dihydroxy-2- isopropylnaphthalen-8-yl) m-ethanone (1), the structure of the compound 4 identified as (4R) -4,5-dihydroxy-3 ,4-dihydro-1 (2H)-naph-thalenone (4), the remaining compounds They were identified as glochidone (2), lup-20 (29)-en-1β, 3β-diol (3), protocatechuic acid methyl ester (5), 4 - hydroxymethyl - 2 - methoxy-phenol (6 ), octadecadienoic Methyl (7), of β-sitosterol alcohol (8), as shown in Fig.1. In the above compounds 1 and 4 are new compounds, 2, 3 and 5 to 7 for the first time from the genus were isolated, and 8 is first isolated from the plant. Using the MTT assay combined with changes in cell morphology under the microscope, and preliminary evaluation of the antitumor activity of K562 cells assigned to the eight compounds. The results showed that five of the compound 1,3 and 5 to 7 having a different degree of anti-tumor activity. The 3 strongest inhibit proliferation of K562 cells IC50 of 6.4μg/mL, and a new compound has strong apoptotic activity. The new compounds 1 and 4, the anti-tumor activity Department first reported. In summary, this study from Tokyo the Pterocarya in separation of eight compounds, 1,3 and 5 to 7, a total of five compounds with a certain degree of anti-tumor activity by activity screening. Which 1 and 4 is a study for the first time discovered a new compound. Fig.1Structuresof1-8isolatedfromPterocaryatonkinesis (Franch.) Dode.
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