Dissertation > Excellent graduate degree dissertation topics show

The Effect of Overexpression of sFrp2 and sFrp3 in the Development of Mouse Molar

Author: LinYunHong
Tutor: ZhangYanDing
School: Fujian Normal University
Course: Developmental Biology
Keywords: sFrp2 sFrp3 Wnt signaling pathway molar ameloblast
CLC: Q954.48
Type: Master's thesis
Year: 2011
Downloads: 4
Quote: 0
Read: Download Dissertation


The tooth development is dependent on the mutual induction between dental epithelium and mesenchymal. Various signaling pathways, in particular, Wnt signaling pathway plays an important role in the process of mutual induction.However,the research about the specific role and mechanism of the Wnt signaling pathway in tooth development remains to be improved.In this essay,we discuss the role of Wnt signaling pathway antagonists sFrp2 and sFrp3 in the development of mouse molar by applying lentivirus-mediated overexpression in dental mesenchymal cells. Real-time PCR results showed that the expression level of sFrp2 and sFrp3 were increased by 270% and 220%, Axin2 expression levels of about 60% reduced.The results above indicated that the efficiency of overexpression of sFrp2 and sFrp3 was good, and the classical Wnt signaling pathway was inhibited. When analysising the transplanted tissue of different days, We discover that the process of mouse molar development was accelerated, the process of ameloblast differentiation and enamel matrix secretion was promoted, the thickness of enamel and the size of the final form of tooth were increased,while the expression of sFrp2 and sFrp3 was accelerated. It was found that, sFrp2 and sFrp3 while over-expression makes the expression of Shh and Bmp4 were reduced by 62% and 52%, indicating that the inhibition of classic Wnt signaling pathway may affect the expression of downstream genes Shh and Bmp4. This experiment proved that, Wnt signaling pathway plays an important role in mouse molar development, especially the development of ameloblast.

Related Dissertations

  1. Expression Change of Signaling Molecule Related Wnt Signal Pathway in Mouse Cleft Palate Induced by Retinoic Acid During Perinatal Stage,R782.21
  2. The cellulose fiber used as structural modification and dissolution behavior,TS102.1
  3. Clinic Study about Protraction of Mandibular Second Molars into First Molar Edentulous Site in Sliding Mechanics with Miniscrew Anchorage,R783.5
  4. A Comparison of Treatment Effect of Molar Intrusion with Micro-implant in Different Facial Skelectal Types,R783.5
  5. The Role of sFrp2 and sFrp3 in Mouse Molar Development,Q954.4
  6. Thermochemistry on the Coordination Behavior of 2-pyrazinecarboxylic Acid with Metallic Elements.,O621.13
  7. Synthesis of Ester-Containing Ionic Liquids and the Interaction in Water,O645.16
  8. Study of Third Molar’s Anatomy,R783.5
  9. The Clinical Research of Distalization of Molars by Micro-implant Anchorage,R783.5
  10. Studies on the Effects and Mechanism of TSPYL5 Anti-oncogene on Development and Progress in Gastric Carcinoma,R735.2
  11. Maxillary second molar anatomy and clinical studies,R781.05
  12. The Three-dimensional Finite Element Analysis on Stress Distribution of Tooth After Root Canal Preparation with Different Instrument,R781.05
  13. The Research on Correlation Methylation of DKK3 and SFRP2 Gene with Breast Cancer in Uigur and Han,R737.9
  14. Effect Analysis of Maxillary Molar Distalization on Class Ⅱ Malocclusion,R783.5
  15. The Expression of Wnt3a in the Process of Adipose Tissue-derived Stromal Cells Differentiated into Dopaminergic Neuron,R329.28
  16. The Effect of Sulindac on Wnt Signaling in Hepatocellular Carcinoma Cell Line SMMC7721 Transfected with HBV X Gene,R735.7
  17. The Three-dimensional Finite Elements Analysis of Maxillary First Molar Being Restored by Inceram-Z All-ceramic Crowns,R783
  18. Clinical Diagnosis and Treatment of C-shaped Root Canals in Mandibular Second Molars,R781.05
  19. Study of the Root Canal System Anatomy of Permanent Maxillary Second Molars in Shandong Province,R781.05
  20. Construction of the Three-dimensional Finite Element Model of the Mandibular Second Molar C-shaped Canal Root System,R816.98

CLC: > Biological Sciences > Zoology > Animal morphology > Animal Embryology ( of animals,animal viviparous learn ) > Tissues and organs and systems ( physical changes )
© 2012 www.DissertationTopic.Net  Mobile