Dissertation > Excellent graduate degree dissertation topics show

The Research of Inhibitory Effects of Sirolimus on Cell Proliferation and Expression of HIF-1α and VEGF Through mTOR Signal Pathway in Human Pancreatic Carcionma SW1990 Cells

Author: WangYu
Tutor: ZhaoQiu
School: Huazhong University of Science and Technology
Course: Digestion within the science
Keywords: Sirolimus Pancreatic Cancer mTOR HIF-1α VEGF
CLC: R735.9
Type: Master's thesis
Year: 2007
Downloads: 127
Quote: 0
Read: Download Dissertation

Abstract


Purpose : Sirolimus is a specific inhibitor of a mammalian target of rapamycin , a recent study found that it has a good anti-tumor effect , but its mechanism is not yet fully elucidated , the experiment through the study of sirolimus cultured human pancreatic cancer cell line SW1990 proliferation and mTOR inhibition of HIF - 1α, VEGF gene explore mTOR as a target in the treatment of pancreatic cancer may be the mechanism of intracellular signal transduction . Method : cultured pancreatic cancer SW1990 cells under hypoxic conditions in vitro , exogenous administration of different concentrations of sirolimus (0.01 nmol/L-100000 nmol / L) to stimulate cell 24h , MTT assay different concentrations of the drug for pancreatic cancer cell proliferation the impact . Semi-quantitative RT-PCR analysis of sirolimus SW1990 cells mTOR, HIF-1α and VEGF mRNA expression . Western blot detection of different concentrations of drugs to stimulate the SW1990 cells of mTOR , HIF -1α and VEGF protein expression changes . Results : sirolimus significantly inhibit the proliferation of pancreatic cancer cells SW1990 , the inhibition rate increased with increasing drug concentration . With the increase in the concentration of sirolimus role of mTOR and VEGF mRNA expression levels in SW1990 cells showed a downward trend , the experimental group and the control group , the difference was statistically significant ( P lt; 0.05 ) ; while sirolimus the concentration level expression of HIF-1αmRNA no significant impact . Sirolimus significantly inhibited the SW1990 cells mTOR , HIF - 1α, VEGF protein expression , the inhibitory effect of the largest group of its concentration maximum . Conclusion : Under hypoxic conditions , the sirolimus via mTOR signal transduction pathway down HIF-1α protein expression in pancreatic cancer SW1990 cells , thereby inhibiting the VEGF gene transcription induced apoptosis , which may sirolimus one of the anti - tumor mechanism .

Related Dissertations

  1. Effects of Angiopoietin-1 on Vascular Angiogenesis Regulator Factor in Human Umbilical Vein Endothelial Cells Cultured with High Glucose,R587.2
  2. Expression and Significance of HIF-1α on the Insulin Resistance during the Injury of Reperfusion of Ischemic Myocardium in Dog Undergoing Cardiopulmonary Bypass,R654.1
  3. Effects of the Applied External Weak Steady Direct Current Electrical Fields on Diastolic Function after Balloon Injury in the Rabbit Abdominal Aorta,R541.4
  4. The Affection and Correlation of VEGF、 SDF-1 to the Homing of Bone Marrow Hemopoietic Stem Cells in Myocardial Infraction Area,R542.22
  5. A Study of the Expressions of MT1FmRNA and VEGFmRNA in Acute Myelocytic Leukemia,R733.71
  6. Expression of NRP-2 and VEGF in Colorectal Adenocarcinoma and Colorectal Adenoma Tissues,R735.34
  7. The Influence of RNA Interference Suppression EphB4 Expression for the PANC-1 Cells of Pancreatic Cancer,R735.9
  8. Establishment and Study of the Cultured Model of Microencapsulated Human Gastric Carcinoma Cells,R735.2
  9. The Molecular Biology of Rh-endostatin Plus Chemotherapy as Adjuvant Treatment for Non-small Lung Cancer,R734.2
  10. Clinical Study on the Expression of VEGF in Liver Metastases of Colorectal Cancer and Its Effect on Prognosis,R735.3
  11. The Effect of Blue Light on α1D Subunit Protein Expression and Secretion Function of Human Retinal Pigment Epithelium Cells in Vitro,R774.1
  12. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  13. VEGF-induced neuronal differentiation into mesenchymal stem cells migration research,R329
  14. Bax and VEGF in endometrial carcinoma and its significance,R737.33
  15. MMP-2, VEGF, CD105, Ki67 in small cell lung carcinoma and prognosis of,R734.2
  16. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  17. Insulin Promotes Proliferative Vitality and Invasive Capability of Pancreatic Cancer Cells Via Hypoxia-inducible Factor 1α Pathway,R735.9
  18. Effect of Leucine on Glucose Uptake in Primary Cultured Skeletal Muscle Cells and the Underlied Mechanisms Mediated by mTOR,R151
  19. The Impact of mTOR Inhibitor RAD001 Combined Gefitinib on Human Non-small-cell Lung Cancer Cell Lines Which Are Egfr-wild and Resistant to Gefitinib,R734.2
  20. The Experimental Research of VEGF Transfected Neural Stem Cells for the Treatment of Cerebral Palsy in the Neonatal Rats,R742.3
  21. Efficacy and Safety of mTOR Inhibitor for Early-stage ADPKD Patients: a Meta-analysis of Randomized Controlled Trials,R692.1

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Pancreatic tumors
© 2012 www.DissertationTopic.Net  Mobile