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The first purpose of a preliminary study of retinoblastoma (Retinoblastoma, Rb) matrix metalloproteinase -2 (matrix metalloproteinase-2, MMP-2) and matrix metalloproteinase -9 (matrix metalloproteinase-9, MMP-9) expression, as well as its Retinoblastoma with the clinical course of the relationship between the optic nerve invasion or not. Further research laminin (laminin, LN) on human retinoblastoma strains (Hxo-Rb44) matrix metalloproteinase-2 and matrix metalloproteinase-9 and its mRNA expression. Method 1 Take 41 cases of patients with Rb paraffin sections using immunohistochemical staining was observed MMP-2 and MMP-9 in the case of Rb tissues. 2 In cultured human retinoblastoma cell line (Hxo-Rb44) respectively added with 0,5,10,20 μg / ml LN serum-free RPMI-1640 medium, cultured for 24h, respectively, by immunohistochemistry SP staining and PT-PCR detection of each experimental group Hxo-Rb44 express MMP-2, MMP-9 and its mRNA levels. 3 using HMIAS-2000 high-resolution color medical image acquisition and processing image analysis system, all data are expressed as mean ± standard deviation (x ± s) that the application SPSS11.5 software for statistical analysis. Differences between groups were compared using t test, the difference was significant with P lt; 0.05 as the standard. Results 1 Rb biopsy in 41 cases in both MMP-2 and MMP-9 expression. There optic nerve tumor infiltrating MMP-2 and MMP-9 expression levels were significantly higher than those without optic nerve invasion of the tumor (P lt; 0.001), in the outside of the eye tumor MMP-2 and MMP-9 expression was significantly higher than the eye stage of the tumor and glaucoma (P lt; 0.01). 2 retinoblastoma LN cells were stimulated intracellular MMP-2 and MMP-9 and its mRNA expression were increased, immunohistochemistry showed 5,10,20 μg / ml LN treated MMP-2 and MMP-9 dyeing the gray values ??were 178.1 ± 11.7,147.9 ± 13.4,114.4 ± 13.6 and 167.0 ± 15.7,140.3 ± 12.0,104.0 ± 10.6, and the control group, P lt; 0.05, LN, and the higher the concentration, the expression of stronger. 5μg/ml, 10μg/ml, 20μg/ml LN treatment groups MMP-2/β-actin, MMP-9/β-actin gray value ratios were 0.181 ± 0.016,0.201 ± 0.021,0.243 ± 0.019 and 0.254 ± 0.023,0.287 ± 0.020,0.320 ± 0.022, compared with control group, the differences were statistically significant (P lt; 0.05), and with the LN concentration increased and increased, showed a dose-effect relationship. Conclusions 1. Rb cells with MMP-2 and MMP-9 expression. MMP-2 and MMP-9 expression level of the optic nerve invasion, and there is a close relationship between the clinical stage, suggesting that the expression level of the tumor invasion and development-related. 2 proved that LN can induce retinoblastoma cells expressed MMP-2 and MMP-9 and its mRNA increased in a dose-dependent. Tips for retinoblastoma LN invasion and metastasis may have promoted.
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