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Expression and Methylation of P27 in Gastric Carcinoma

Author: Li
Tutor: LiuHongJun
School: Shandong University
Course: General Surgery
Keywords: gastric carcinoma p27 immunohistochemistry methylation
CLC: R735.2
Type: Master's thesis
Year: 2008
Downloads: 62
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Abstract


Objective:To investigate the methylation status of p27 in gastric carcinoma, analysis the different of the p27 expression in DNA,RNA and protein,and compared it with that of tumor-sideward mucosa and that of normal gastric tissues,to find out the deactivation mechanism of p27,and study the relationship between p27 biological characteristics and proliferation characteristics,to find out the cause that affect the cell cycle.Method:Immunohistochemistry was applied to examine the expression of p27 in 49 cases of human gastric carcinoma,20 cases of tumor-sideward mucosa and 10 cases of normal gastric,and use MSP to determination the 5 ’CpG island methylation status of p27.Results were compared with clinical pathologic parameter such as histology type、lymphatic metastasis and clinical stage.Result:1.Expression of p27 protein:①Positive immunostaining with the antibody was observed in the nuclear.②57.14%(28 of 49)of cases had expression of p27 in the tumor cells,and in 95.00%(19 of 20) of cases of the tumor-sideward mucosa,and 100%of cases(10 of 10)of the normal mucosa it were present.There was a significant positive correlation between gastric carcinoma specimens and normal mucosa specimens and tumor-sideward mucosa(p＜0.05).③There was no significant diference between normal gastric group and tumor-sideward mucosa group(P＞0.05), but both were higher than cancer group(P＜0.005).④Tumors with lymph node metastases(38.71%) had loss p27 protein expression than those without metastasis (88.89%)(P＜0.05).The p27 expression betweenⅠ+ⅡandⅢ+Ⅳstage,the difference was significant(82.35%vs 43.75%)(P＜0.05).The positive of p27 in high and middle degrees of differentiation were significantly higher than that in low degree of differentiation(P＜0.05).The positive rates of P27 decreased with clinical stage,the poor differentiation,and lymph nodes metastasis(P＜0.05).p27 protein expression had no relation to the patients’ age,sex,tumor size and location.2.Methylation of p27 gene:①The positive product of PCR is 198bp.②Using MSP we found that the status of p27 gene promoter regions CpG island have been methylated.The mathylation rate is 48.98%(24/49) for gastria cancer,25%(5/20) for tumor-sideward mucosa,and 0(0/10) for normal mucosa.There was a significant positive correlation between gastric carcinoma specimens and normal mucosa specimens(p＜0.05).③There was no significant diference between normal gastric group and tumor-sideward mucosa group(P＞0.05),and no significant diference between tumor-sideward mucosa group and tumor group(P＞0.05).④The mathylation rate of p27 gene with lymph node metastases(61.29%) are higher than those without metastasis(27.78%)(P＜0.05).And there is no relation on patients’ age,sex,tumor size and location.3.The relationship between the methylation of p27 gene and the expression of p27 protein:The methylation rate in positive expression of p27 protein is 35.71% (10/28),in negtive expression is 66.67%(14/21).The result show:in all stages of disease,the expression of p27 protein was negatively correlated with methylation of p27 gene.(P＜0.05).Conelution:The p27 protein expression decrease or loss may be involved in development of gastric cancer,p27 gene methylation could lead to the loss of p27mRNA transcription.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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