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Background: neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, motor neuron disease, etc.), traumatic diseases (brain, spinal cord and peripheral nerve damage), cerebral hemorrhage, cerebral infarction, and inflammation of the nervous system, tumor after surgery nerve function repair Dengjun involved in nerve regeneration. But there has been no effective treatment. In recent years, with the deepening of the technical studies of carbon nanotubes (CNTs), it was found that CNTs especially in neuroscience have potential applications in the study of the mechanism of the nerve cells in vitro. It may be used as a graft in vivo induces the regeneration of the spinal cord and the brain tissue damage, can also be used as external skeletons of cells induced directional growth of axons. At the same time be able to adjust the axon branch formation, and ultimately the formation of the complex connection between the synapses. As a new biological materials, if CNTs can induce nerve regeneration, then you can think hemiplegia, paraplegia, peripheral nerve injury and even neurodegenerative disease provides a new method of treatment. Objective: To understand the CNTs promote functional recovery of the sciatic nerve incomplete injury. METHODS: The experiment: to determine the therapeutic effect of CNTs on incomplete sciatic nerve injury functional recovery. 24 adult Kunming mice were randomly divided into two groups, all mouse sciatic nerve were forceps injury. Be 1mg/kg bw / day for the experimental group (13) of the amino-functionalized multi-walled carbon nanotubes (MWNT) be the saline 0.5ml/kg bw / day by intraperitoneal injection intraperitoneal injection of the control group (11), determination of the first day and the fifteenth day of the mouse sciatic functional index (SFI larger the value the worse). Experiment 2: Determine the CNTs on incomplete sciatic nerve injury in the role of dose-response and time effects. Take 47 adult Kunming mice were randomly divided into four groups. The all mouse sciatic nerve were forceps injury, and the establishment of a control group, be the saline 0.5ml/kg weight / day by intraperitoneal injection experiment three groups be 0.1mg / body weight / day, respectively, 0.5mg/kg body weight / day, 1mg/kg body weight / day of the amino function of the MWNT intraperitoneal injection, prior to the experiment after the experiment, 1,7,10, and 15 days were measured mouse SFI. Experiment: Determine the MWNT whether damaged sciatic nerve tissue can be reached. Eight hours to take the injury of the sciatic nerve in mice by intraperitoneal injection MWNT specimens in a transmission electron microscope. Results: The experimental: SFI value in the first day of the two groups was no significant difference (P = 0.373), the fifteenth day of the experiment mice sciatic functional recovery was significantly better than the control group, the SFI value difference was significantly (P = 0.003). Experiment II: CNTs are obviously incomplete sciatic nerve injury therapeutic effect of dose-response and time effect. Compared to the dose-response: 0.1mg / body weight / day MWNT intraperitoneal injection group and the control group was not significantly different (P = 0.16), and 0.5mg/kg body weight / day, 1mg/kg bw / day MWNT intraperitoneal injection group and the control group. than the differences are significant (P = 0.002, P <0.001). Time effect: 7,10,15 differences are significant (P <0.001), seven days and 10 was not significantly different (P = 0.276) compared with the first day, and the first 15 days and 7,10 day differences are significant (P <0.001, P = 0.001). Experiment: the observed transmission electron microscope MWNT present in the damaged sciatic nerve Shi Wan cytoplasmic. Conclusion: CNTs can quickly reach the damage to the sciatic nerve and sciatic functional recovery promoted increased with dose and the passage of time and the enhanced role.
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