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The Study of Sperm Acrosomal Morphology and Function

Author: WangWenHui
Tutor: LiuYuSheng
School: Anhui Medical University,
Course: Obstetrics and Gynaecology
Keywords: Sperm Semen routine Papanicolaou stain Acrosin activity Gelatin substrate film method Acrosome reaction Upstream method Sperm morphology
CLC: R698.2
Type: Master's thesis
Year: 2008
Downloads: 155
Quote: 0
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Abstract


Objective 1. To explore the gelatin substrate membrane test acrosin activity laboratory quality control. 2. Explore the relationship between sperm acrosome morphology and sperm acrosin understand different acrosome Teratospermia the acrosome activity, as well as relations with IVF (in vitro fertilization, IVF) fertilization rate. The transmission electron microscope to understand the link between sperm morphology and function. Sperm morphology and function checks, choose the right conception. The method according to the World Health Organization (WHO) standards, samples should be taken in abstinence 3-7 days after sperm retrieval in the center chamber through masturbation, take semen in plastic containers immediately after collection still in the 37 ° C waters boxes liquefied , conducted a routine examination of the semen, semen achieve IVF assisted reproduction standards included in the specimens of choice, morphological examination, divided into two groups (IVF and half-ICSI (of the half intracytoplasmic sperm injection) group). Liquefied sperm by upstream method in the modified Papanicolaou stain test line sperm acrosin activity of gelatin method tests. The transmission electron microscope examination of 11 cases of semen analysis. Results 1. Temperature of gelatin stretched, the appropriate sperm density and film storage gelatin substrate film method determination of acrosin activity stability, improve the reliability. . The upstream treatment after the sperm to achieve a survival rate of 98.7%, for the examination of sperm morphology and function to lay the foundation of a living cell. Normal sperm morphology rate lt; 5% of patients undergoing IVF progesterone than normal sperm morphology ≥ 5% of patients with fertilization rates reduce P lt; 0.05, statistically significant. 4 acrosome irregular shape or containing multiple small vacuoles fertilization rate was significantly reduced. The acrosomal contains multiple small vacuoles acrosin activity decreased. Sperm morphological examination, the acrosome irregular proportion in the comparison of normal fertilization and fertilization barriers statistically significant (P lt; 0.05), transmission electron microscopy light microscope, the structure and function of different abnormal sperm Analysis. Conclusion 1. Upstream Screening spermatozoa sperm function tests, better response to sperm fertilization. Through quality control, gelatin method test sperm acrosin activity can become a stable, more reliable method of determination of sperm function. Optical microscope under top body vacuoles transmission electron microscope images of nuclear vacuoles, dense nuclear vacuoles reaction degree of maturity of the sperm. 4 by semen examination the (rortine semen analysis RSA), Pap stain analysis of morphological, Determination of acrosin activity, some abnormal semen for in vitro fertilization ability of prediction.

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Men 's sexual dysfunction > Male infertility
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