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Influence of Beta-catenin Antisense Oligodeoxynucleotide on Cells Proliferation and Sensitivity to Paclitaxel of MCF-7 Cell Lines

Author: SongQi
Tutor: HuangZuo
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: MCF-7 cells Breast Cancer Antisense oligonucleotides Apoptosis Taxol
CLC: R737.9
Type: Master's thesis
Year: 2007
Downloads: 20
Quote: 0
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Abstract


Objective To investigate the β-catenin antisense oligonucleotide (ASODN) breast cancer MCF-7 cell proliferation , and Taxol ( Paclitaxel P) combined with inhibition of MCF-7 cells . Of different concentrations of β-catenin ASODN and missense control (SODN), blank control role in MCF-7 cells 48 hours later, the proportion of apoptotic cells was determined . To a final concentration of 10μmol / L in ASODN SODN and blank control cultured cells 10 days after the calculation of each colony formation rate . MTT assay join ASODN, SODN, paclitaxel and blank control in cultured cells by different combinations in culture for 24 hours , 48 hours by reverse transcription polymerase chain reaction (RT-PCR) detection of β-catenin mRNA expression after 72 hours of incubation calculated for each group cell viability . MCF-7 cell apoptosis proportion with β-catenin ASODN role concentration increases slightly elevated with SODN, blank control group , a significant difference ( P lt; 0.05 ) . ASODN group average colony formation rate ( 12.5%) and SODN group ( 34.0% ) and the control group (37.0 %), the difference was statistically significant ( P lt; 0.05 ) . MTT assay and fluorescence quantitative RT-PCR results ASODN group in combination with paclitaxel inhibition of cell growth rate and β-catenin mRNA expression relative value is the same in both single ASODN group or paclitaxel alone group exists significant differences . Conclusion β-catenin antisense oligonucleotide inhibition of MCF-7 cell proliferation , promote apoptosis , and with the combined effects of paclitaxel significantly inhibited the expression of β-catenin mRNA and the proliferation of breast cancer cells .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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