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Objective: To observe the β-elemene carbon tetrachloride hepatic fibrosis of TGF-β1 and α-SMA, Col-I, MMP-2, MMP-9mRNA expression affect, so as to explore the β elemene anti-hepatic fibrosis of the mechanism of action.: CCl4 subcutaneous injection of male Wistar rat model of hepatic fibrosis induced with β-elemene 0.1ml/100g of dose daily intraperitoneal injection of 8 weeks, stained with hematoxylin - eosin and collagen fibers (Masson) staining of rat liver pathological changes, enzyme power detected liver function, the SP immunohistochemistry assay in liver tissue α-actin (α-SMA), transforming growth factor-β1 (TGF-β1), I type I collagen (Col-Ⅰ) expression changes, the sample alkalinehydrolysis detection of liver tissue hydroxyproline (HYP) content by reverse transcription polymerase chain reaction (RT-PCR) determination of MMP-rat liver tissue expression levels of MMP-9mRNA integral optical density ratio (V / B), V / B values ??reflect the organizations of MMP-2, MMP-9 mRNA expression levels of high and low results: after 8 weeks of treatment administered normal group, model group, the control group, the treatment group liver tissue collagen fiber area percentage were 1.22 ± 0.24,7.47 ± 0.81,5.57 ± 0.78,4.33 ± 0.48, treatment group vs the model group (P lt; 0.01), the treatment group vs the control group (P lt; 0.01), the degree of liver fibrosis grading compared with the model group gradually improved, significantly reduced collagen fiber area occupied; enzymatic indicators ALT, AST, ALB model group and treatment group were 254.88 ± 70.18 vs 208.47 ± 85.70; 414.42 ± 138.11 vs 330.92 ± 107.40; 26.54 ± 1.59 vs 28.47 ± 1.11, P lt; 0.05; measured in liver tissue hydroxyproline OD ratio were 0.162 ± 0.048,0.410 ± 0.145,0.273 ± 0.088 0.186 ± 0.120, the treatment group vs the model group (P lt; 0.01); positive area ratio of collagen type I were 3.022 ± 0.553,9.998 ± 1.431,7.554 ± 0.914,4.587 ± 1.008 treatment group vs model group (P lt; 0.01 ), the treatment group vs the control group (P lt; 0.01); expression of α-SMA in liver tissues were 0.968 ± 0.281,5.605 ± 1.315,4.914 ± 0.893,3.172 ± 0.542, the treatment group vs the model group (P lt; 0.01); TGF-β1 expression in the liver tissue were 1.026 ± 0.208,5.653 ± 0.9,4.702 ± 0.627,2.868 ± 0.554, the treatment group vs the model group (P lt; 0.01), the treatment group vs the control group (P lt ; 0.05). liver tissue measured by RT-PCR the MMP-2mRNA integral optical density ratio, respectively (1.0124 ± 0.1257,1.9353 ± 0.1447,1.5885 ± 0.2963,1.3205 ± 0.3875 vs normal group model group P lt; 0.01 ; model group vs treatment group P lt; 0.05); the MMP-9mRNA integral optical density ratios (0.1684 ± 0.0745,0.5137 ± 0.0918,0.3833 ± 0.1605,0.3178 ± 0.1204, model group vs normal group P lt; 0.01; model group vs treatment group P lt; 0.05) Conclusion: β-elemene carbon tetrachloride liver fibrosis in rats with antagonism by reducing hepatic fibrosis process of TGF-β1, α-SMA, COL-I MMP-2 and MMP-9mRNA expression to inhibit hepatic stellate cell activation and deposition of extracellular matrix (ECM) degradation, and thus reversed the fibrosis formation.
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