Dissertation > Excellent graduate degree dissertation topics show

The Effect of the Formation of Biofilm on the Susceptibility of Pseudomonas Aeruginosa

Author: LiZuo
Tutor: JiangHanDong
School: Qingdao University
Course: Internal Medicine
Keywords: Pseudomonas aeruginosa β—lactamsase biofilm MBEC real time PCR
CLC: R516
Type: Master's thesis
Year: 2008
Downloads: 188
Quote: 1
Read: Download Dissertation

Abstract


Objective: To investigate susceptibility of Pseudomonas aeruginosa biofilm strains generating different types ofβ-lactamase to antibacterials in different culture media. And investigate the effect of Pseudomonas aeruginosa biofilms on different genotypesβ-lactamases.Methods: Select P. aeruginosa isolates generateing expanded spectrumβ-lactamases （ESBL）, Cephalosporinaseβ-lactamase （AmpC） or Metalloβ-lactamases （MBL）, and set up the biofilm moulds in three kinds of media: L-Broth（LB）, Tryptic Soy Broth（TSB） and Mueller-Hinton Broth（MHB）. Biofilms were established using a modification of the Calgary biofilm device. This technique involved the generation of biofilms on plastic pegs attached to the lids of 96-well microtiter plates; Determine minimal inhibiting concerntration（MIC） and minimal biofilm elimination concerntration （MBEC） to three kinds of antibacterials, which are typically used against Pseudomonas aeruginosain cilinic: Ciprofloxacin. Ceftazidime and Imipenem, with micro-dilution and MBECTM-Device method. The clinical Pseudomonas aeruginosa strains known genotype of beta-lactamase of TEM and CARB were collected and induced by three antimictobials: Ciprofloxacin、Ceftazidime and Imipenem to generate beta-lactamases after the biofilms were built. And the differet expression of beta-lactamases were determined in above status by polymerase chain reaction （PCR）. The results were statistical analysized with t test and U test.Results: There was no difference in the susceptibility for planktonic strains to antimicrobials, in LB, MHB and TSB media in statistics （P >0.05）; While after the formation of biofilms,the biofilm strains could be resistant above 64 times MIC of planktonic strains to Ceftazidime , 16～128 times MIC of planktonic strains to Imipenem, and 4～32 times MIC of planktonic strains to Ciprofloxacin, respectively. And after the formation of bacterial biofilms. there existed different effects of culture media on bacterial susceptibility to Ciprofloxacin and Imipenem different media in statistics: LB<MHB<TSB （P < 0.01） ; The noninduced biofilm cells and induced by CIP、CAZ and IMP all produced much more beta-lactamases, and the quantity of TEM-beta-lactamase were 1.77, 13.85, 16.37, 34.58 times more than that of noninduced planktonic cells, and CARB-beta-lactamase were 2.94、9.53、10.84、21.90 times more than that of noninduced planktonic cells, respectively. The amplitude of TEM beta-lactamase produced by induced planktonic cells were 2.04、3.31、8.65 times more than that of noninduced planktonic, and the amplitude of TEM-beta-lactamase produced by induced biofilm cells were 7.82、9.25、19.54 times more than that of noninduced planktonic cells. The amplitude of CARB-beta-lactamase produced by induced planktonic cells were 3.51、5.88、8.77 times more than that of noninduced planktonic cells, and the amplitude of CARB-beta-lactamase produced by induced biofilm cells were 3.24、3.68、7.45 times more than that of noninduced planktonic cells.Conclusion: Different microenvironments cannot influence the susceptive of Pseudomonas aeruginosa planktonic cells to antimicrobials, but influence biofilm cells. The formation of biofilm could influence the ability of Pseudomonas aeruginosa strains to produce different beta-lactamase; and the ability to produce TEM-beta-lactamase was stronger than CARB-beta-lactamase.

Related Dissertations

  1. Cloning and Expression of CHS and CHI Genes and Their Regulation on the Accumulation of Flavonoids in ’Cara Cara’ Navel Orange (Citrus Sinensis Osbeck) and ’Guoqing NO.4’ Satsuma Mandarin (Citrus Unshiu Marcow),S666.4
  2. Biological Effect of Rare Earth Element and Genetic Transformation on Arnebia Euchroma(Royle) Johnst.Cell,S567.239
  3. Select of Biofilm Formation Mutants and Cloning and Primary Function Analysis of tatC Gene from Pseudomonas Fluorescens Strain 7-14,S432.4
  4. Treatment of High Concentration Oil-Bearing Wastewater in Food Industry with Membrane Biofilm Reactor (MBR),X792
  5. Study on Screening of Bacterial Quorum Sensing from Plant Extraction and Their Inhibitions on Biofilm Formation,Q93
  6. The Effect of Polysaccharide-producing Rhizobium Sp. Q32 on Silicate Minerals Weathering and Its Mechanism,Q93
  7. In Vitro Biofilm Formation of Enterococcus Faecalis in Starving Phase and Antibacterial Effect of Sodium Hypochlorite,R780.2
  8. Virulence Factors of Enterococcus Faecalis Expression in the Biofilm of Starvation Phase and after Two Root Canal Conventional Drugs,R780.2
  9. Induced Resistance to Low Temperature and Plant Disease by Bacterial Fermentation Fluid for Soybean,S435.651
  10. The Relationship between Aciduric Virulence Factor F-ATPase Expression in Biofilms and Caries,R781.1
  11. Real-time PCR and RT-PCR analysis of ordinary Enterococcus faecalis esp gene differences,R440
  12. Technical Improvement Study on Wastewater Treatment with High Ratio of Industrial Wastewater by Anaerobic/Aerobic Activated Sludge Process,X703
  13. Effects of 60Coγ Radiation Exposure on Expressions of CDKN1A, FABP4 and HSL in Human Lymphocytoblast,R144
  14. Effect of Chitooligosaccharides on Streptococcus Mutans Biofilm,R780.2
  15. Influence of Pseudomonas Aeruginosa Autoinducer of Las on Murine Macrophage in Vitro,R378
  16. Study of Pipeline Internal Corrosion of Sulfate-reducing Bacteria in the Presence of Carbon Dioxide,TE988.2
  17. Genetic Variation in CD226 Gene and Systemic Lupus Erythematosus,R593.241
  18. Complex three-dimensional electrode - Removal of biofilm reactor Experimental Study of nitrate in drinking water,X703
  19. Extraction of wool keratin and its application,TS195.56
  20. Culture and its effect on aerobic granular sludge wastewater treatment research HMX,X703
  21. The Research of Resistance and Related Genes in Biofilm Bacterial from Double-J Tube,R378

CLC: > Medicine, health > Internal Medicine > Infectious disease > Bacillus of infectious diseases
© 2012 www.DissertationTopic.Net  Mobile