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Objective: To explore the total saponins Dioscorea improvement in metabolic markers in obese rats. 8: SPF level of pregnant rats, litter size 96, starting at 2 days after birth, newborn rats, divided into two groups, one subcutaneous injection of sodium L-glutamate (L-MSG) 4g/kg.d that the MSG model group; group injected with saline served as normal controls. Continuous 7d. 21d after weaning, regularly records the weight, observed body shape changes. Two groups have been fed a normal diet. Selection of weight gain 20w age the larger 30 successful modeling male rats were randomly divided into Dioscorea saponins groups, Avandia group, model control group, the normal group of seven rats were included in this study together. Weighed, the amount of body length calculation Lee's index overnight fasting, inner canthus venous plexus blood measurement of high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), triglyceride (TG), insulin (Ins), glucose (BG). Dioscorea saponins group were given a the Dioscorea total saponins dimethyl sulfoxide, the Avandia group given Avandia dimethyl sulfoxide, model control group, the normal group were given distilled water, dimethyl sulfoxide gavage to total gavage time 8w measured weight, the amount of body length calculation Lee's index measuring HDL-C, TC, TG, Ins, BG, rats were sacrificed, weighed perirenal and retroperitoneal fat weight. Specimens from liver, kidney, pancreas, adipose tissue is saved to liquid nitrogen, and specimens from the liver in 10% neutral formalin fixed. HE staining of liver tissue morphology observed. MSG group results: 1 16w age rats compared to Lee's index and the normal group difference was statistically significant (P <0.01), 20w age between the MSG groups and the normal group when various biochemical markers HDL-C, TC, TG, Ins, BG and Lee's index difference were significant sex (P <0.01) ② Dioscorea total saponin group, the Avandia group Rats were before and after treatment the biochemical markers HDL-C, TG, Ins, BG differences were statistically significant (P < 0.01), but the TC change in the Avandia group is not obvious (P> 0.05), while in Dioscorea saponins group differences were significant (P <0.05). Model group TG, Ins, BG, HDL-C was significantly increased (P <0.01), the TC no significant change. Liver HE staining observation prompted Dioscorea saponins can improve fatty liver, almost back to normal. The Avandia group also improved, but the effect is not as good as Dioscorea saponins obvious. Dioscorea saponins the group cultural Avandia group compared after administration biochemical indicators no significant difference (P> 0.05). Conclusion: The total saponins of Dioscorea to reduce the obese rats INS, TC, TG, FBG, and increased HDL-C, to reduce IRI. And our experiment found that the total saponin of Dioscorea lower TC's role seems to be superior to Avandia, liver HE staining showed that total saponin of Dioscorea can significantly improve fatty liver, its effect than Avandia. This is the active ingredient of the research Dioscorea opened new avenues for the treatment of insulin resistance.
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