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Male germ cells cultured in vitro research is a hot topic in the field of reproductive biology research . Mammalian germ cells cultured in vitro has made ??great progress , the basic realization of the proliferation and differentiation of spermatogonial cells cultured in vitro , spermatocytes differentiate into sperm cells in the process of meiosis . But not sperm cells cultured in vitro differentiation process of sperm . Relative to mammalian cell culture , crustacean cell culture started relatively late , and culture focused on shrimp hemolymph , heart and ovarian proliferation ability testis cultured in vitro studies reported less . Chinese mitten crab in male germ cells study adherent cell rate , survival rate and RNA / DNA ratio for physiological and biochemical parameters were measured several different osmotic pressure (600,700,800,900,1 000,1 100 mmol / L) and the pH ( 6.8,7.0,7.2,7.4,7.6,7.8 ) cultured in vitro under the growth conditions of the male reproductive cells . Pointed out that the 1 000 mmol / L for optimum osmotic pressure of the cultured cells , adherent cultured cells under the conditions of this osmotic pressure , survival , RNA / DNA . Cultured cells suitable pH range is 7.2-7.4, in the pH conditions of adherent cells , survival , RNA / DNA are larger . On this basis , the observed growth of the cells in the cell culture and tissue culture . Found that the survival of cells in the cell culture time is short, the ability to split low ; tissue culture, the cells survive longer, proliferation ability , can form a partial monolayer organization block center . In addition, the the add removal mitten crab eyestalk hemolymph of male germ cells cultured in vitro . The results show that hemolymph removed 48 h after eyestalk can promote cell growth , RNA / DNA maximum value , but you can not start and promote cell division , and removed 24 h 0 h blood lymph no statistically significant difference .
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