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A549 cells and Hep-2 cell surface sugar chains type identification and its characteristics combined with the H5N1 avian influenza virus

Author: HuangLi
Tutor: YinHongChao
School: Beijing Union Medical College
Course: Pathology
Keywords: HSN1 virus Airway epithelial cells Sialic acid -a2, 3 - galactose Sialic acid -a2, 6 - galactose Cell death
CLC: S852.65
Type: Master's thesis
Year: 2010
Downloads: 22
Quote: 0
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Abstract


Background: The highly pathogenic avian influenza virus H5N1 can infect humans and cause fatal respiratory disease . Once the avian influenza virus in interpersonal communication capacity will likely cause a human influenza pandemic . Usually avian influenza virus to the affinity of the human upper respiratory tract cells , may be to limit the propagation factor in the H5N1 virus between people . However , the H5N1 avian influenza virus of the human respiratory tract tropism and pathogenic mechanism is still not clear . Therefore, the study of the H5N1 virus invasion of airway epithelial cells of human is important to understand the spread of avian influenza virus and pathogenic significance . Objective: To establish the Hep-2 cells and A549 cell model to study the H5N1 avian influenza virus tropism and adhesion of human respiratory role . Method: lectin staining and flow cytometry detection of influenza virus A549 cells and Hep-2 cell surface sialic acid receptor SAa2, 3Gal and SAa2 expression of 6Gal . H5N1 virus labeled with FITC and indirect immunofluorescence detection of the H5N1 virus to enter cells . Western blotting method to analyze the efficiency of the virus into A549 cells and Hep-2 cells . MTT assay was used to detect the H5N1 virus treated cell survival situation . Results : A549 cells and Hep-2 cell surface large number SAa2 of 3Gal chain , but rarely SAa2, 6Gal chain content . Hep-2 cell surface SAa2 3Gal receptor expression levels than A549 cells . The H5N1 virus is able to enter the A549 cell and Hep-2 cells , and the H5N1 virus tropism stronger in A549 cells . Compared with A549 cells , Hep -2 cells of the H5N1 influenza virus -induced cell death is more sensitive . H5N1 induced cell death is a non - of Caspases dependent , and has nothing to do with PI3K activation .

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Basic Veterinary Science > Animal Microbiology ( Veterinary Microbiology, ) > Livestock Virology
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