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Study on the Preparation of Hydrolyed Porcine Blood Cells and Its Feeding Value

Author: HuGang
Tutor: WangChunWei
School: Wuhan Polytechnic University
Course: Animal Nutrition and Feed Science
Keywords: porcine blood cells neutral protease degree of hydrolysis soluble nitrogen in trichloroacetic acid weaned piglets growth performance index of physiolgical chemistry
CLC: S816
Type: Master's thesis
Year: 2010
Downloads: 38
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Abstract


The paper was conducted to determine the optimal conditions of proteinase to hydrolyze procine blood cells, and to evalulate the feeding value of hydrolyed spray-dried animal blood cells(SDBC). the test is divided into three parts.Expt.1: Study on the conditions of proteinase to porcine blood cellsThis test selected neutral protease hydrolyzed porcine hemocyte.By single-factor test, the effects of hydrolysis conditions on the hydrolysis rate were considered , which were the reaction temperature、pH、enzyme/substrate、the concentration of substrate and the reaction time. According to the orthogonal experimental design , the hydrolysis condition of porcine hemocyte by neutral protease treatment are as follows:reaction temperature55℃、pH 7.5、enzyme/substrate 5000U/g、concentration of substrate 10% and the reaction time 3hunder these conditions, the degree of hydrolysis was 22.03% and the content of soluble nitrogen in trichloroacetic acid was 63.83%.Expt.2:Study on the decolouring technology of enzymatic hydrolysis of procine blood cellsThe effects of activated bentonite content pH、temperatures、decolour times and additive amount on decolourzation of enzymatic hydrolysis of procine blood cells were studied in this paper. The results showed that discolouration conditions were followed: reaction temperature40℃、pH 4.0、additive amount of activated bentonite0.25%、and the decolour times 20min. under these conditions,decolorant rate is 98.67% and protein loss ratio are 24.29%.On this basis, using saccharomyces cerevisiae carry off their debitter.Add 5% of the saccharomyces cerevisiae response to 5h.By sensory evaluation debittering effect is good. Expt.3: Effects of enzymatic hydrolysis of procine blood cells on growth performance and biochemical in weaned pigletsThe trial choose 24 crossbred weaned piglets (Duroc×Landrace×Yorkshire) were randomly allotted to four three dietary treatments, with six replicate pens per treatment and one piglets per pen.Dietary treatm- ents included control group (3.5%fishmeal) testgroupⅠ(procine hema- tocyte)、test groupⅡ(enzymatic hydrolysis of procine blood cells)、test groupⅢ(enzymatic hydrolysis and debitterize of procine blood cells). The trail divide up two phase, the 1st is 0-14d, the 2nd is 15-28d. At the end of the trail, blood samples from anterior vein were collected to determine the biochemical of serum.The results indicated that:1: at phase 1st,compared with the control group, ADG of the test groupⅡwere increased 2.36%, but test groupⅢwere decreased (P> 0.05); compared with the test groupⅠ, ADG of he control group、test groupⅡ、test groupⅢwere increased 12.84%、15.64% and 10.72%(P< 0.05), but feed conversion were decreased 11.98%、10.18% and 10.18%(P< 0.05). on systemic resolutions,compared with the test groupⅠ,ADG of the control group、test groupⅡ、test groupⅢwere increased 3.34%、5.48%and 1.71%(P> 0.05); compared with the control group, feed conversion of the test groupⅠ、test groupⅡ、test groupⅢwere increased 7.48%、5.19% and 7.48% (P< 0.05). 2: compared with the test groupⅡ,BUN of the control group、test groupⅠ、test groupⅢwere decreased 24.96%、32.33% and 19.26%(P< 0.05); compared with the control group,AKP of the test groupⅠ、test groupⅡ、test groupⅢwere increased 4.72%、10.36% and 5.94% (P> 0.05); contents of TP were no differences(P> 0.05); compared with the control group, LDH of test groupⅠ、test groupⅡ、test groupⅢwere increased 7.91%、2.81% and 0.96%(P> 0.05).

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