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Epidemiology Inspection of Immunosuppressive Viral to Large-scale Farms Meat in Yantai

Author: TangMaoHeng
Tutor: ZhuRuiLiang
School: Shandong Agricultural University
Course: Veterinary
Keywords: ALV CIAV REV Testing Epidemiology PCR
CLC: S858.31
Type: Master's thesis
Year: 2010
Downloads: 35
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Abstract

ALV, CIAV, REV, ReoV were the major immunosuppressive virus in current chicken flocks. These immunosuppressive virus can cause primary infection in different extent. Even more, these virus can make other pathogens easily infected, and then make chicken immunity descend, chicken body immunosuppressive and effect of vaccine fall. Current reports showed that these virus existed in the investigated chicken flocks and Co-infections were serious. The breeder flocks were infected by them and then the 1d chicken flocks were infected vertically which made the death rate of commercial chicken flocks increase. So infections of immunosuppressive virus were the potential menace to poultry industry at present, and bring mass economic loss for poultry industry at present.This research divides into two parts:Part one: ALV, CIAV, REV, ReoV blood serum epidemiology investigation.This experiment has the representative meat to the Yantai area to plant the chicken group the chicken leukemia (AL), chicken infection poor hemopathy (CIA), the chicken Reticulo-endotheliosis multiplication sickness (RE), the chicken to shout the intestines arc to get sick Reovirus (Reo) 4 kind of immunosuppression sickness to carry on the blood serum epidemiology investigation. Mainly uses agar-agar diagnosis methods and so on proliferation experiment, ELISA experiment to carry on the serum antibody examination. And titre and the bacterium masculine gender rate has carried on the examination to immunity suppressant infectious disease masculine chicken group’s ND the HI, simultaneously carries on the diagnosis to the Yantai area above 4 kind of immunity suppressant epidemic disease’s clinical case, has counted in near 3 years the Yantai area these 4 kind of epidemic disease’s infection and the morbidity situation. The result indicated that the immunity suppressant infectious disease infects the chicken group to cause its immunity ability to be low, ND HI titre remarkable somewhat low. The immunity suppressant epidemic disease’s existence is constitutes this local meat to plant the chicken house chicken group immunity defeat, the epidemic disease popular primary factor.Part two: Epidemic Investigation of infection of REV, ALV, ReoV and CIAV in meat-type flocks in yantaiThe tissues samples were collected from 617 birds in I、II、III chicken flocks wich suspected to be infected by immunodepressive virus in Yantai. The DNA was extracted for hybridizationin Dot-blot with probes of REV, ReoVand CIAV. At the same time, we investigated Avian leukosis virus subgroup p27-shedding in cloaca swabs from collected birds using ELISA method. The result indicated that these virus existed in the investigated chicken flocks and co-infections were serious. Single infection and co-infection increased by degrees from broiler breeders to commercial chicken in diseased farms. II was detected as the region where occur ALV frequently most of all. The checkout ratios of REV in III is higher than other regions. The result indicated that existing in wide range and co-infection of REV, ReoV, ALV and CIAV in chickens are the important causations for high incidence opportunistically pathogenic diseases and descent of poultry production performance in Yantai.Part three: Immunity suppressant virus separate and appraisal and PCR diagnosis method establishmentThis experiment plants in the chicken house from Yantai area some meat AL, CA, RE, the Reo 4 typical symptom sickness chicken’s pathological change to organize to separate one poison respectively, through virus’s isolated culture and the PCR examination determination for the above four kind of viral distinction naming is ALV-YT01, CIAV-YT01, REV-YT01, ReoV-YT01. Will separate four kind of virus vaccinate on 11th the age SPF chicken embryo to present the typical characteristic value clinical symptoms and the pathological change separately, separates poisonous to be able by the respective antiserum neutral, 5 week age SPF chicken vaccination separation cytotoxin may produce the immune body, the electron microscope observation separates in the poisonous cell culture medium to have the massive viral granule respectively, directs the thing using specificity PCR to be possible to increase to the respective estimate length viral nucleic acid fragment.Using the PCR examination method which establishes to Yantai area other meats plants chicken house four kind of immunity suppressant epidemic disease’s suspected case each 40 to carry on the examination, the detection rate respectively is 37.59%, 42.13%, 31.47%, 27.79%, compares the examination rate with the traditional viral parting method examination is the enhancement greatly.According to the chicken leukemia (AL), chicken infection poor hemopathy (CIA), the chicken Reticulo-endotheliosis multiplication sickness (RE), the above four kind of virus which the half separated out has carried on the appraisal, and has established the corresponding PCR examination methods, providing the theory and technology for the control disease.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Poultry > Chicken
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