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Isolation and Characterization of an Antifungal Protein from Black Sesame

Author: CuiXiaoHui
Tutor: TangYiXiong
School: Chinese Academy of Agricultural Sciences
Course: Molecular biology and biochemistry
Keywords: Black sesame seeds Antibacterial proteins Purification Antibacterial activity
CLC: S565.303.4
Type: Master's thesis
Year: 2000
Downloads: 301
Quote: 2
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Abstract


Many plants have antibacterial proteins endogenous constitutively expressed . These antimicrobial proteins are not only strong antimicrobial activity and a broad spectrum antibiotic , more and more important in the prevention and treatment of plant diseases . After the first of these antibacterial protein purification can be directly used as biochemical reagents , followed by determination of antimicrobial protein sequence in the original plant cloning the gene , which is an important source of disease resistance genes in plant disease resistance genes . Consider these antimicrobial proteins are derived from plant endogenous gene expression , and so from the point of view of the security of the transgenic plants more easily accepted by the masses , and to allay their concerns on genetically modified food psychology . This experiment using the low pressure by Bio-Rad Econo chromatography systems , the Bio -CAD high pressure liquid chromatography system , out of the anion by Fastflow Q anion column chromatography and molecular exclusion chromatography , column chromatography and POROS Q 20 is extracted from the black sesame strong resistance to a wide range of fungi antibacterial protein, which is different fungal inhibition IC 50 different fungi different initial detection of strong resistance to the common bean wilt the their the IC 50 is less than 28μg/ml ; cotton anthrax , Trichoderma also have significant activity , but significantly increased IC 50 ; does not resistant cotton yellow dwarf and wheat Gibberella . The protein in the polyacrylamide gel electrophoresis single tape, and a molecular weight of about 31 kd . Further experiments found that this protein is resistant to proteinase K , but not high-temperature heating antimicrobial activity was lost soon after . Extracted from black sesame endogenous expression of efficient , broad-spectrum antimicrobial protein domestic and foreign publications has not been reported . Some of the other features of the protein is still in its understanding of its antibacterial mechanism needs further study , and use it to better serve mankind .

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CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Sesame seeds ( Zhi Ma )
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