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Grape seedless gene molecular markers

Author: YangYingJun
Tutor: WangYueJin；ZhouPeng
School: Northwest University of Science and Technology
Course: Pomology
Keywords: Grape seedless gene Molecular markers Southern blot
CLC: S663.1
Type: Master's thesis
Year: 2000
Downloads: 238
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Abstract


The 1.18bp specific primers in the grape varieties verify 18bp In this study, the detection grape seedless gene -specific primers , the presence or absence of 37 grape varieties of nuclear and non-nuclear , non-nuclear gene molecular diagnostics using RAPD technique . Results demonstrate 18bp specific primers were designed and synthesized by UBC-269-484 sequence does have detected grape seedless gene function . Cloning , sequencing and SCAR 2.RAPD tag tag conversion of 18bp generate approximately 590bp specific fragment was amplified recovery, cloning , and bi-directional sequencing , the entire sequence of the fragment , the sequence of a total length of 569bp . PC-gene software analysis , design and synthesis of the two pairs of specific primers , P 1 P 3 and P 2 P , P 1 < / sub > P 2 and P 3 sequence : 5'CTC ATCTTC TTG ATG GTG AT3 ', 5 'GAC CAG TCT GCA CTT ACA GT 3', 5'AAG TGAAGA ACA TCA TTA AGA AC 3 ', and all cultivars tested for PCR amplification, two specific fragment of about 530bp and 500bp , the fragments or not accurately distinguish between nuclear and non-nuclear grapes . Successfully convert RAPD markers into SCAR marker . The 3.RAPD mark and SCAR markers in the breeding of grape seedless applications using RAPD markers and SCAR markers of the outstanding results of the five nuclear × seedless hybrid combination of a total of 165 individuals were fruit nuclear and non-nuclear traits pre- options to achieve the purpose of molecular marker-assisted selection of grapes seedless . 4.Southern blot detection grape seedless gene grape seedless RAPD marker gene sequence PC-gene software restriction site analysis , the choice of the Hind III and Spe Ⅰ endonuclease double digestion of the recombinant plasmid to obtain a 310bp fragment was recovered and purified DIG - labeled made ??hybridization probes for the detection grape seedless gene existence ; using the probe successfully seedless , red Globe seven fine grape varieties Southernblot analysis to distinguish between grape varieties of nuclear and non-nuclear purposes .

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CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Berries > Grapes
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