Dissertation > Excellent graduate degree dissertation topics show

Protective Effect of GDNF on Spinal Cord Motorneuron in Incomplete SCI and Its Mechanism in Rat

Author: SongHaiTao
Tutor: JiaLianShun
School: Second Military Medical University
Course: Surgery ( bone )
Keywords: Spinal cord injury Animal models GDNF NTFs Gene Expression ACP ChE Ca2 ~ MEP SEP
CLC: R651.2
Type: Master's thesis
Year: 2000
Downloads: 84
Quote: 0
Read: Download Dissertation

Abstract


In the past two decades, a lot of research work has confirmed damage to the central nervous system plasticity, can be regenerated; regeneration speed and quality but also in the role of neurotrophic factors (NTFs) have significantly enhanced neurotrophic factor is the support of neurons and save damage to neuronal survival role has become a \GDNF is a neurotrophic factor in recent years (1993) found that the cloned gene, belonging to transforming growth factor-β superfamily member, is a specific strongest dopaminergic neurons nutritional factors, retired in the body sympathetic, feeling the survival of neurons play a support role. It is widely expressed in the central nervous system, peripheral nerves, and other sports system organization, especially in the embryonic and injury the higher peripheral nervous expression, suggesting that tissue development and repair nerve damage from the role of nutrition. Recently found that GDNF on motor neurons in vitro also salvage its death role, but the role of spinal neurons has not been reported. SCI after expression of NTFs its receptor increase the performance of self-protection role. After SCI outer peripheral target tissue and damage to the nervous element can produce is limited, and trace NTFs, but because of the damage to axons and neurons lost contact with the target tissue, NTFs no longer axonal transport to the neuron cell body; combined with a large number of nerve yuan competing NTFs, which makes not get sufficient NTFs neuron programmed cell death occurs, provides a theoretical basis for the exogenous GDNF treatment of SCI. In this study, the main method: 1. The the rat AJlen incomplete spinal cord injury model 2. After subarachnoid functional evaluation of the spinal cord (1) were treated with GDNF, SAL, at different times: Rivlin inclined plate rats climbing angle: (2) spinal cord electrophysiological examination: including MEP and SEP; (3) spinal cord morphology examination: CHE ACP staining the average gray positive particles (MG) and the equivalent circle diameter (ED) as indicators observed neuronal survival. 3. Respectively at different times after the injury to the injured spinal cord, the expression of different periods GDNFmRNA after injury was determined by semi-quantitative RT-PCR method. 4. Observed after spinal cord injury, spinal cord water content, intracellular free calcium content and topical application of GDNF impact of this trend. The main results of this study are as follows: the l second academic Yin Wen l% gift stand leg of the rat spinal model. 2. Spinal cord tissue after spinal cord injury in rats demonstrated for the first time that high expression GDNFmRNA, peak expression after injury, 72, J '. 3. Spinal cord stained the positive granules MG and ED to the ACP and CHE staining, as a reference, found that GDNF can effectively alleviate the increase in the activity of ACP and CHE activity weakened prove the element has a protective effect of GDNF on spinal cord injury and motor nerve, and its role in peak In the first 10 days after injury. 4. ivlin oblique pole experiment confirmed the GDW significantly increased in rats climbing angle, 10 and ZI days after injury, respectively, than the SAL group increased GDNF can effectively improve hindlimb muscle strength, improve spinal cord function. 5. Electrophysiological results show that after spinal cord injury SEP and MEP crest reduce the latency, GDNF can alleviate this change. 6. After spinal cord injury moisture content increased intracellular calcium content of GDNF treatment after spinal cord water content and reduce the level of intracellular calcium, but has not yet reached normal levels. The results of this study suggest that: 1. Alien g hit animal model of hindlimb function change to reflect the degree of spinal cord injury; 2. GDNF from the expression is minimal (normal) to high expression, indicating that after spinal cord injury will require a lot of GDNF supports application of exogenous GDNF treatment of spinal cord injury provided a strong theoretical foundation, and also verify Wrathall secretion of endogenous neurotrophic factor, spontaneous save neurodegenerative death this assertion, again confirmed NTFs active ingredients for the treatment of SCI. 3. GDNF injured rat spinal cord neurons have a protective effect, can effectively improve the hind limb motor function; 4. Mechanism the mouth DN'F improved spinal cord function, may be related to reduce nerve cells * wide influx.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  3. Study on Synthesis and Physiological Function of N-carbamoyl-glutamate,R914
  4. Functional Analysis of Two Rice Nitrate Transporter Genes OsNRT1.1a and OsNRT1.1b,S511
  5. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  6. Study of Chicken Δ~6 Fatty Acid Desaturase Gene’s Promotor Region Polymorphism and Gene Expression,S831
  7. The Expression of CPV-2 VP2 Gene in SF9 and Development of Indirect Elisa for Serum Antibodys,S852.65
  8. Comparison of Gene Expression Data Cluster Methods and Gene Network Construction for Phytophthora Sojae Genes,S435.651
  9. Functional Identification of Two Tomoto Phosphater Transporter Genes LePT1 and LePT2 in Rice,S511
  10. Analysis for Intron 1 and 2 of Rice Transformation to Promoter Activity,S511
  11. Research on Characteristics of Growth, Development and Material Metabolism of Flue Cured Tobacco Leaves in Huili, Sichuan,S572
  12. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  13. Functional Characterization of Wheat Na~+ /H~+ Antiporter TaNHX2 and Study of TaNHX2 C- Terminus Domain,S512.1
  14. Identification and Analysis of miRNA and Flower Development Specific Genes in Wheat,S512.1
  15. Study on Ca2+/H+ Antiporter Avtivity and Its Gene Expression of Apple,S661.1
  16. Molecular Characterization, Expression, and Promoter Methylation Status of the Bovine Synaptonemal Complex Protein 3 Gene,S823
  17. The Deposition Rule and Gene Expression Involved in Yolk Cholesterol in Different Layer Breeds,S831
  18. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  19. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  20. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  21. The Molecular Mechanism Intervention of Selenium on the Learning and Memory Injury by Drinking Water Fluorosis,R599

CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
© 2012 www.DissertationTopic.Net  Mobile