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The Separation of Growth-promoting Bacteria (Phosphate-solubilizing Bacteria & Azotobacteria) from Local Mangrove and Formulation Research

Author: GongZuoBin
Tutor: LiuJunAng；HeXueXiang
School: Central South University of Forestry Science and Technology
Course: Forest Protection
Keywords: Rhizosphere promoting bacteria Microcapsules Formulations Mangroves
CLC: S718.8
Type: Master's thesis
Year: 2009
Downloads: 40
Quote: 0
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Abstract


The plant rhizosphere beneficial microbial agents research and application technology is attracting much attention of the international research project, which is important in the rhizosphere settlers (or colonization), the development of new formulations help to solve this problem. With the deepening of the the rhizosphere microbial agents research, rhizosphere microorganisms on tree survival and growth of the importance of increasing understanding of the world, applied research is generally subject to the attention of the world. The thesis for the study of the coastal areas of Guangdong local mangrove forest root interpersonal promoting bacteria studied the local mangrove Thuringia occasion promoting bacteria, to carry out its separation screening, medium optimization and capsule formulation developed to make it become a survival high, easy for industrial production and transport of new agents. The local mangrove Thuringia occasion promoting bacteria separation screening. Guangdong Nansha Wetland Park taken back to the wild mangrove Avicennia marina seedlings for material separation has dissolved P, solid N capacity strains screened from the local Mangrove Thuringia International Isolation and Screening of the Guangdong Provincial Academy of laboratory dissolved P bacteria 3 Zhu Number: P7 #, 07PCK, 07pqwp1-5 #; solid N bacteria 4 kinds of, ID to: NGQQ ② The D, NGWB ③ The ④ B, MLN2 #, 07Nhs-7 # for the strains tested, at room temperature potted conditions study 7 strains the of red trees Lam seedlings (Guangdong Nansha Wetland Park) vaccination effect, the results show: wood Lam solid N-inoculation of seedling height increased by 20.4% to 31.8% compared with the control, the biomass ratio was increased by 19.4% to 21.6% seedling height and biomass wood Lam significance analysis showed that height, compared to the biomass with the control were highly significant value; the dissolved P inoculation wooden the seedlings height ratio was increased by 22.8 percent to 28.5 % biomass ratio control increased from 19.4% to 22.6%, seedling height and biomass of wood Lam analysis showed significant height, biomass compared with the control were highly significant value. Root Colonization of promoting bacteria detection In pot experiment process, the solution P bacteria and the solid N mycorrhizal colonization of the order of magnitude of the number of colonies reached more than 109,, to promote mycorrhizal Ministry colonization good. The rhizosphere to promoting bacteria medium conditions optimization. Screened out of view of the different environments promoting bacteria on the adaptability of the medium is not the same, the study for the test laboratory screening promoting bacteria strains, the single factor promoting bacteria growth impact study results show that promoting bacteria medium conditions: salinity 15 ‰, pH 8.0, nutrient source C source is glucose, N source is peptone. The local mangrove Thuringia interpersonal growth promoting bacteria capsule formulations developed. Trials developed a new formulation of the local mangrove promoting bacteria - micro-capsules, sodium alginate selection of wall material, the use of orifice - coagulation bath legal system to do the capsule formulation. Test microcapsules glue formulations optimized morphology through the production of microcapsules of 15 different formulations of glue, the hardness comparison, screening out the best glue formulations: alginate (2.5%): The broth mixed = 3:1. Trial will save the the microcapsule formulations (dry goods, wet goods) and liquid dosage forms (4 ℃, -20 ℃) ??strains survival compared, results showed that the retention of room temperature liquid dosage forms of bacteria worst two months after the survival rate of less than 100,000, the number of viable cells tested dissolved P bacteria and solid N bacteria only 5.59x104, 7.37 × 105, the capsules dry goods room temperature to save 2 months, the survival rate can still reach 10 million, for try dissolved P bacteria and solid N bacteria viable count of 11.37 × 106,10.13 × 107. Inoculation effect under potted conditions at room temperature of the test screening promoting bacteria produced large capsules, the microcapsules and conventional liquid dosage forms (mixed bacteria, single bacteria) of mangrove candel seedlings (Guangdong Nansha Wetland Park), The test results show that the inoculated promoting bacteria inoculant candel seedling height increased by 12.6% to 23.0% compared with the control, the biomass ratio was increased by 16.2% to 17.5%. Significant difference analysis showed that the inoculated promoting bacteria inocula candel seedling height compared with the control were highly significant differences in biomass compared with the control, except NGQQ ② D single inoculated candel candel seedling height and biomass seedlings were highly significant differences. Each dosage form of the pro-mycorrhizal colonization in good condition, especially microcapsules best, and the capsules dry goods carry, easy to transport than liquid formulations, its superiority is obvious.

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CLC: > Agricultural Sciences > Forestry > Forestry basic science > Forest Biology > Forest Microbiology
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