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In this study, Basidiomycetes Pleurotus genus Pleurotus (Pleurotus sajor-caju) as experimental material , obtained by submerged fermentation , Pleurotus mycelium and fermentation broth . Mycelium by ethanol extraction solvent to give the mycelium ethanol extract by solvent extraction . The activity was detected , the mycelium crude extract its extraction proposition bacterial antifungal activity is weak . Fermentation broth of ethyl acetate and n-butanol fractional extraction , showing strong antibacterial activity , and the use of a silica gel thin layer chromatography , column chromatography on silica gel and Sephadex LH 20 means of gel chromatography , separation of the component . Tracking experiments by their biological activity from having good anti-bacterial anti-fungal active ingredient , wherein component EPscl , preferably the Npscl inhibitory effect against bacteria tested . Component EPsc4 - 1 loquat anthracnose mycelial growth and spore germination has strong inhibitory activity , to the IC50 respectively to reach 0.119mg/mL and 0.323mg/mL . GC-MS analysis found component EPsc4 - main active ingredient may naphtho [ 1,2 - d ] imidazole . Observed by electron microscopy, component EPsc4 1 loquat anthracnose mycelial morphology and structure . Scanning electron microscopy , hyphae irregular enlargement and constricted , obvious deformity , mycelial growth was significantly inhibited . Transmission electron microscopy results show that the hyphal cell wall irregular thickening of the cell protoplasm is uneven , larger cavity formation , increased vesicles , part of the mycelium tissue necrosis .
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