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The application of molecular markers and DNA fingerprinting technique seaweed germplasm

Author: ShiJinFeng
Tutor: ZhangTingZuo；WengManLi；WangBin
School: Northwest University of Science and Technology
Course: Tree Genetics and Breeding
Keywords: Seaweed Germplasm Molecular markers DNA fingerprinting
CLC: S968.431
Type: Master's thesis
Year: 2001
Downloads: 283
Quote: 2
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Abstract

Nori is an important economic red algae, all over the world have a wide range of distribution, in our seaweed output value ranked first in the seaweed. Has long been used in the production of seaweed strains mostly from wild provenance. Seaweed germplasm mainly uses traditional morphological methods, is still in a relatively backward stage, can not meet the demand for seaweed production and scientific research. Germplasm problem has long been an important limiting factor for serious problems plagued seaweed production, but also the development and use of seaweed resources and problems to be solved in the production. Has become imperative in order to prevent or reduce the use of substandard clones to seaweed production losses caused establish an accurate and efficient laver germplasm. In the present study, the main application of our four categories (P. yezoensis, Porphyra yezoensis, altar seaweed P.Haitanensis second half of the seaweed P.Katadai var. Hemiphylla and less refined seaweed P.Oligospermatangia,) 15 seaweed clones filamentous using RAPD genetic diversity analysis, which used 8 Operon primers amplified stable repeatable map product polymorphism as high as 70-97%, 15 clones clustering based on RAPD results 3 group, this clustering results with traditional taxonomic status agreement. We selected eight good stability and reproducibility typical polymorphic bands, used to build Porphyra germplasm using DNA fingerprinting, eight bands from primer OPJ-18 and OPN-02 amplification results. Basis eight band appears in the gel pattern or not the RAPD amplification results are recorded and converted into a language recognized by the computer for, building a schematic diagram of DNA fingerprinting and the computerized DNA fingerprinting, in each of the Spectrum clones with DNA fingerprinting can be distinguished from other clones, so we developed a computerized fingerprint-based seaweed clones germplasm new method using the method Jiangsu the laver seed field identified 10 Porphyra Department, through the national identification, as China's first batch of seaweed clones enter the country seaweed germplasm bank, and then based on the design of specialized computer application software for laver germplasm PhGI (Porphyra Germplasm Identification), from 8 The eight clones specific RAPD amplification products recycling clone, they are useful RAPD markers. Five RAPD markers by sequencing, based on the sequencing of the 5 'end of the base sequence of five pairs of SCAR-PCR primers were designed and synthesized by trial and error and comparison, optimization SCAR-PCR reaction conditions, the RAPD clones K9401 and Y9502 specific markers successfully converted into SCAR markers and STS markers. SCAR markers have been used to guide the production of seaweed practice, and has achieved significant production benefits. This is the first time this new molecular marker introduced by the higher plants, the field of molecular biology and applied to the algae field of molecular biology and the success of the instance. These specific molecular markers Laver clonal germplasm identification, lasting and reasonable utilization and protection of property rights is of great significance.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquaculture technology > A variety of seafood, animal and plant breeding > Algae farming > Red algae > Laver
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