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Preliminary Study on the Compatibility between Metarhizium Anisopliae and Chemical Pesticides

Author: YeXiaoZhen
Tutor: SongZhang
School: Fujian Agriculture and Forestry University
Course: Forest Protection
Keywords: Metarhizium anisopliae Chemical pesticides Chemical fungicides Extracellular protease Chitinase Compatibility
CLC: S476.12
Type: Master's thesis
Year: 2009
Downloads: 70
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Abstract


Metarhizium anisopliae (Metarhizium anisopliae) is an important the insect parasitic fungus, is widely used in microbial control of pests. The advantages of effective prevention of Metarhizium and chemical pesticides, the determination of 10 kinds of commonly used chemical insecticides on Metarhizium anisopliae conidia germination, colony growth, sporulation, and detection of Metarhizium anisopliae and chemical insecticide mix, the Metarhizium anisopliae extracellular protease and chitinase activity, trying to clarify its synergy of chemical insecticides on Metarhizium anisopliae. Simultaneous determination of chemical fungicides on Metarhizium anisopliae conidia germination. This effective use of biocontrol fungi have a guiding role. The main results are as follows: the test 10 kinds of chemical pesticides affects both Metarhizium anisopliae and the inhibition weakened with the decline of the concentration of pesticides. Different pesticides on Metarhizium anisopliae conidia germination inhibition of colony growth and sporulation of the same. , Bifenthrin and cypermethrin most of Metarhizium anisopliae conidia germination smaller, avermectin Ma201 Ma203 Ma205 conidia germination inhibition rate smaller, Avi chlorbenzuron the Ma208 strains inhibition the weakest. Avi diflubenzuron, methomyl and phoxim smaller the Metarhizium anisopliae colony growth inhibition and negative inhibition rate, low concentration levels, indicating that low concentrations of Avi diflubenzuron Methomyl and phoxim promote Metarhizium anisopliae colony growth. Negative inhibition at low concentration levels, the majority of pesticides (such as malathion, bifenthrin, trichlorfon, methomyl, avermectin and insects hydrazide) Metarhizium anisopliae sporulation role, indicating that the low levels of pesticides that may contribute to Metarhizium anisopliae sporulation. In addition, different strains of Metarhizium anisopliae with an insecticide sensitivity is not the same. Wherein Ma208 conidia more sensitive to the majority of pesticides, but relatively insensitive to cypermethrin. Ma336 strain colony growth by chemical pesticides, other strains of colony growth was less affected by pesticides. Ma202 Ma103 strain sporulation influenced by chemical pesticides, to a lesser extent by other strains. Chemical insecticides on Metarhizium anisopliae production chitinase has a great influence. Three strains treated with chemical pesticides, tested most strains of Metarhizium anisopliae chitinase activity has reduced, especially higher chitinase activity (Ma103, Ma337, Ma208); Ma205 chitinase activity of the strain did not change significantly; Ma202 Ma207 strain chitinase activity mention slightly Board. Chemical pesticides impact on the the Metarhizium anisopliae production protease. After chemical insecticide-treated, tested most of the strains of Metarhizium anisopliae protease activity has decreased; Ma201, Ma203 strain protease activity did not change significantly; only Ma202, Ma207 strain protease activity increased slightly. Different chemical fungicides on Metarhizium anisopliae conidia germination, and decreased with the decline in the concentration of the fungicides. Myclobutanil and Zineb inhibitory effect of the spores weakest spore germination rate of processing, showed that the smaller myclobutanil and Zineb germination of Metarhizium anisopliae conidia. And several other fungicides significantly inhibit the germination of conidia, in particular chlorothalonil and thiophanate-methyl, the conidial germination inhibitory rate reaching almost 100%. Different Metarhizium anisopliae strain sensitivity to the same chemical fungicides are not the same.

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CLC: > Agricultural Sciences > Plant Protection > A variety of control methods > Biological control > The use of microbial pathogens > Insects, fungi
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