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In recent years, Chinese medicine pesticide residues has been widespread concern. After the the of Crops in the Pesticide Residue Limits in Foods to enter the the human body, the Once the the dose of poisoning is reached, it will caused on the the the patient's body serious injury. In order to safeguard the people's safety of drug use, the countries around the will More of pesticides and varieties of traditional Chinese medicines included in the detection range, With the increase in the kinds of of of pesticide, they are required and constantly improve the the of pesticide residues in detection method, while the existing some kind of or a particular type of traditional Chinese medicine detection of pesticide residues in you methods have been unable be to meet the for the requirements of the the development of reality!. In this article I want to research and the establishment of a Chinese herbal medicine of pesticide residues in rapid detection method to be applied to the four types of commonly used pesticide organochlorine, organophosphate and pyrethroid and carbamate residues determination. In the the test, By examining the to extract the type of of the solvent, ultrasonic extraction the temperature of the and ultrasound extraction time, etc. affect the the the factors that of the extraction efficiency, and ultimately determine in order to the 1% of the acetic acid-acetonitrile was added dropwise to the mixture solution for the extraction solvent, 4 ℃ under the ultrasound after the extraction of a period of 20mmin, dispersive solid phase extraction method the process of on the purification a the previous of Processing on. Experimental groping, and ultimately established the following gas-phase MS conditions. GC conditions: DB-17MS capillary column (30 m × O.25mm 0.25um); temperature program: initial temperature to 60 ° C and maintained for 1 min, with a heating rate of 30 ℃ / min to 130, then to 10 ° C / min heating rate to 200 ° C and a heating rate of 1.4 ° C / min to 237 ° C and a heating rate of 2 ℃ / min to 241 ° C and a heating rate of 1.4 ° C / min to 243 ° C, and then to 3 ° C / min heating rate to 249 ° C, and finally to 300 ° C to 12 ° C / min of heating rate to maintain 17.6mmin; inlet temperature of 290 ° C; column flow to 1.0 mL: splitless injection, the injection volume was 1 ul . Mass spectrometer conditions were: the the temperature of the transmission line for the 280 ° C, the a temperature ion source of 230 ° C, The temperature of the Quadrupole for the to 150 ℃ ionization mode for the electronic bombardment ionization (EI), electron energy: 70eV; The solvent delay time is 8min; data acquisition mode for the sub-period of time selected ion monitoring mode (SIM). Test proved that inter-the in the selected ion monitoring under the mode of, the the So the characteristic ions of the 53 kinds of pesticide separated well from; of all the pesticide in the to showed a good linear relationship in the the within the scope of of 0.0102-31.0 mg / L, correlation coefficients were all in the more than 0.99: detection limit in the 0.003- 0.3 ng between the limit of quantification in the 3.3 to 123.3μg/kg: when the sample addition amount in the range of 3.3 to 493.2μg/kg, recoveries and relative standard deviation were 76.2-109.8% and 2.0-12.4% of the inter-; when the the the Sample Add the amount of is in the the 6.7 ~~ 986.4μg/kg between the when, the the the recoveries and relative standard deviation, respectively, in the between 74.3-105.3% and and 1.9-10.5%; When the sample Add the amount of when between in the 13.2 1972.8μg/kg , recoveries and relative standard deviation, respectively, in the the range of 79.2-105.1% and for between the 1.7-10.6% of, the 53 kinds of pesticide, only omethoate the the the recovery rate of of the and cis-heptachlor epoxide is lower than 80%. Methodological study results show that the analysis method is accurate, simple, fast, and can be used in Chinese herbal pesticide multi-residue rapid determination. The method used in licorice root, skullcap, honeysuckle, March 7, Sophora, Tianma, astragalus, ginseng and Cork 9 of 66 samples of common Chinese herbal medicines detection of pesticide residues. In from the to medicinal herbs to see has been detected, the 66 medicinal herbs has 23 detection pesticide, accounting for 35% of the total the total amount of; starting with checking out of a pesticide of view, the number of 53 kinds of pesticide there are 16 kinds was detected, of which the \There are eight kinds: alpha-BHC, gamma-BHC, o, p'-DDT, p, p'-DDE and p, p'-DDT, fenvalerate, cypermethrin and methidathion phosphorus ; \phosphorus, phorate, and triazophos. \The isofenphos banned pesticides in the cultivation of Chinese herbal medicines. \The established in this paper the at the same time of the than the 53 kinds of pesticide residues in the the currencies in the Chinese herbal medicines, processing and Method for determination of, quick and easy, can be achieved the a a large number of of the multi-residue of the of medicinal herbs pesticides rapid detection of, for the the Chinese herbal medicines one exports and a limited number the development of standards to provide a reference information.
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