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Ultrasound activated hematoporphyrin on isolated human melanoma (LiBr), gastric cancer ( 7901 ) Preliminary study on the killing effect

Author: ZhangFengYue
Tutor: LiuQuanHong
School: Shaanxi Normal University
Course: Zoology
Keywords: SDT SONODYNAMICS Antitumor Hematoporphyrin MTT Apoptosis
CLC: R73-36
Type: Master's thesis
Year: 2001
Downloads: 39
Quote: 0
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Abstract

Malignant tumors, especially human life is a threat to the safety of one of the diseases, diagnosis and treatment of tumors has been the focus of research at home and abroad. Among the many methods of diagnosis and treatment of cancer, in particular, the use of focused ultrasound ultrasound ultrasound and developed methods antitumor growing concern. Ultrasound is an elastic material medium mechanical wave. As a form of physical energy, ultrasound in the material in the mechanism of the media can be divided into thermal effects, cavitation and mechanical mass effect, its intensity and the frequency and intensity of the ultrasound. Hematoporphyrin and its derivatives are a class of low toxicity and a substance having a strong photosensitivity in the normal endogenous porphyrins in the body material is an intermediate product of heme synthesis, is acetic acid and glycine as a substrate, in the mitochondria enzyme catalyzed synthesis. Hematoporphyrin and its derivatives have specific accumulation in tumor tissue and retention properties. Shinichiro Umemura other Japanese scholars proposed the use of ultrasonic activation of hematoporphyrin (Hematoporphyrin) and its derivatives (HpD.Hematoporphyrin derivatives) to produce anti-tumor effects SONODYNAMICS (Sonodynamic Therapy, SDT), is the use of a photosensitive material hematoporphyrin morpholine selectively retained in tumor tissue and by activated anti-tumor characteristics, and particularly focused ultrasound ultrasound in biological tissues have a strong penetration ability to focus on non-invasive deep tissue characteristics. The method and the other two at home and abroad have promising ultrasound therapy: high-intensity focused ultrasound (HIFU) compared to surgery and ultrasound heat therapy to overcome their deficiencies in clinical, so much in antitumor attention has become a very promising anti-tumor approach and has been developing rapidly in recent years. Many scholars this method has been carried out some research on tumor cells in vitro and in vivo xenograft small animals has accumulated some information, but the information obtained remains insufficient, the approach has some limitations, from the reach clinical applications there is a great distance. In order to make an early SONODYNAMICS used in clinical research at home and abroad on the basis of the National Natural Science Fund Project \the amount of hematoporphyrin, the use of SDT method for both isolated human tumor cells (melanoma LiBr, gastric cancer cell 7901) conducted a study on killing effect by MTT method to filter out the best intensity ultrasound treatment, the best treatment time; and using the SDT method, in a certain frequency focused ultrasound probe, combined with a certain amount of hematoporphyrin, hematoporphyrin of ultrasonic activation induce tumor cell apoptosis done a preliminary study. And get the main conclusions are as follows: 1. Using the MTT assay on fixed-frequency focused ultrasound (00KHz) activates blood Ye Lin killing two kinds of human tumor cells in vitro (LIBf3 7901) the best ultrasonic intensity, the role of screening time parameters, further confirmed the MTT method parameters SONODYNAMICS The filter is a better way, and once again confirmed the different types of tumor cells to different frequency ultrasound, ultrasonic intensity and type of different reactions to different treatment times. 2. Using this method the existing ultrasound device gained LIBr, 7901 Best ultrasonic intensity of tumor cells, the role of the time parameters for the study SONODYNAMICS accumulated a certain amount of information. *) At a frequency of 1. IMHz focused ultrasound, the 7901 cell lines ultrasound energy output was 0.06. / Cm '. Cell viability was negatively correlated with processing time, processing time of 180 seconds, the 4 hours to reach S7% mortality. ② In the frequency is 1. IMHz focused ultrasound, Lisr cells in the output energy of 0.06. Eight Kuang, the treatment time 180 seconds, 4'J \\ time after reached 71% mortality. m at a frequency of 1. IMHZ focused ultrasound, intensity of 0.06. / Cm ', the processing time of 60 seconds and 90 seconds, after 8, J', the first test of the cells measured LIBr obvious apoptosis. 3. In the above study found that low-intensity ultrasound on human tumor cells in vitro have a certain role in promoting growth, which is reported in the literature a certain intensity ultrasound may promote tumor growth of tumor-bearing mice are similar, but the specific mechanism needs further study. 4. Bio-based radical theory, harmony kinetic mechanism of apoptosis, in previous studies, based on the combination of ordinary optical microscope, fluorescence microscopy, transmission electron microscopy observation of ultrasonic treatment changes in cell structure, and the use of DNA fragments agarose gel electrophoresis to detect ultrasound activated Ye Xi induce tumor cell apoptosis phenomenon. SONODYNAMICS proposed anti tumor cells and promote cell death inducing apoptosis has two modes, under certain conditions, one of the dominant mode, but does not exclude the effect of another mode. As the complexity of the biological effects of ultrasound and SONODYNAMICS singlet oxygen and free radical theory has not yet fully elucidated, these two models have not drawn the distinction between quantitative indicators. Information obtained from these studies for future in vitro, in vivo experiments provide important parameters and basis for further promoting early SONODYNAMICS have some clinical significance.

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