|
Objective: To study Endostar (endostar, rhEndostatin recombinant human endostatin ) on the C57BL / 6 mouse lung metastatic melanoma immune micro- environment CCL12 ( chemotactic factor 12 ) , CCL21 ( chemokine 21), Foxp3 ( forkhead - like / winged helix transcription factor ), TGF-β1 ( transforming growth factor β1) mRNA and HIF-1α ( hypoxia inducible factor ) , VEGF ( vascular endothelial growth factor ) protein expression . Whether the analysis endostar can alleviate the immunosuppressive tumor microenvironment . Thus provide new support for the theory for Endostar clinical applications . : The method of intravenous injection of C57BL / 6 mice lung metastatic melanoma model . Tumor-bearing mice were randomly divided into the treatment group and the saline control group of Endostar intervention . Intervention Endostar 20mg/kg/d, 100μL, once every 10 days , the control group received saline . Weighed every other day to monitor weight change . Mice were sacrificed after the last intervention , the lung tissue . Under a dissecting microscope to count lung metastases number . Immunohistochemical detection of lung tissue HIF-1α, VEGF protein expression . RT-PCR and real-time PCR detection CCL12 , CCL21 , Foxp3 of TGF-β1 mRNA expression levels . Results : ⑴ endostar to the increase in both the weight of the drug group and the control group of mice , but the difference was not statistically significance ( P gt ; 0.05) . ⑵ Endostar treatment group reduce the number of lung metastases in mice compared with the control group , the difference was statistically significant ( P lt; 0.05 ) . ⑶ Endostar the medication set of HIF-1α, VEGF expression was decreased compared with the control group , the difference was statistically significant ( P lt; 0.05 ) . ⑷ Endostar medication group CCL12 mRNA expression increased , CCL21 mRNA, Foxp3 mRNA, TGF-β1 mRNA expression reduced compared to the control group , the differences were statistically significant ( P lt; 0.05 ) . Conclusion : Well , can improve hypoxic state , reduce immunosuppression to reduce the transfer of C57BL / 6 mice lung metastatic melanoma . Provides theoretical support for the for Endostar clinical applications .
|