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Experimental Study on the Inhibition of Corneal Neovascularization with Bevacizumab and Their Ultrastructure

Author: LinFang
Tutor: GaoXiaoWei
School: Shihezi University
Course: Ophthalmology
Keywords: Bevacizumab Cornea Neovascularization Vascular endothelial growth factor
CLC: R772.2
Type: Master's thesis
Year: 2009
Downloads: 9
Quote: 0
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Abstract


Objective: To investigate bevacizumab corneal neovascularization and vascular endothelial growth factor (VEGF) in corneal and ultrastructure . Methods : disk experiments 128 Wistar rats were randomly divided into four groups , adopt prepared alkali burn corneal neovascularization model . Not normally burn group 32 , 1 , injection of 0.1 ml saline group ( 32 ) every other day under the bulbar conjunctiva after burns , burns the next day subconjunctival injection of bevacizumab 0.1 ml group ( 32 ) , 3 , burns after every other day subconjunctival injection of dexamethasone 0.1 ml ( 32 ) . Observed turbidity of the rat cornea after alkali burns in a slit lamp microscope ; macroscopic measurement neovascularization length : HE staining of tissue biopsy microvessel counts ; transmission electron microscopy to observe the ultrastructural changes ; Immunohistochemical detection of VEGF protein expression ; the CD34 marker neovascularization , microvessel count method under the microscope to study the corneal neovascularization and inhibition . Results : The treatment group compared with the control 3,7,10,14 days group the corneal opacity Light ( P lt; 0.05 ) ; number of new blood vessels formed in 14 days compared with the control group was less ( P lt ; 0.05 ) experimental group neovascularization microvascular reduce the number of VEGF protein expression decreased , with a significant difference . VEGF expression in the the cornea damaged area within the cytoplasm of infected cells , the time and location of corneal neovascularization consistent . Bevacizumab and dexamethasone can effectively inhibit corneal neovascularization , reduce VEGF expression within the cornea , no statistically significant difference between the two rat cornea ultrastructure no addition to the subconjunctival injection of bevacizumab and dexamethasone The other significant change after burn . Conclusions: VEGF is an important corneal neovascularization factors , changes and corneal neovascularization parallel ; certain dose bevacizumab , dexamethasone inhibit corneal neovascularization , reduce VEGF expression within the cornea , and both of corneal the ultrastructure no significant drug toxicity . .

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CLC: > Medicine, health > Ophthalmology > Eye fiber membrane disease > Corneal diseases
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