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Survivin Antisense Oligodeoxynucleotide Loaded Liposome Enhanced the Apoposis of Hepatoma Carcinoma Cell Line HepG2 Induced by Adriamycin

Author: ZhangSheQin
Tutor: MiaoXiongYing
School: Central South University
Course: General Surgery
Keywords: Hepatic carcinoma Liposomes Survivin Antisense oligonucleotides
CLC: R735.7
Type: Master's thesis
Year: 2010
Downloads: 18
Quote: 0
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Abstract


Objective: liver (hepatocelular carcinoma, HCC), is one of the most common malignant tumor high degree of malignancy and poor prognosis, and a serious threat to human life and health. Currently, surgery is still the treatment of HCC preferred and more effective way. Chemotherapy is an important means of adjuvant therapy in HCC, but the toxicity to normal cells and tumor cell resistance to chemotherapy facing two problems. Studies have shown that, Survivin is the strongest apoptosis inhibitor discovered to date, and has the dual function of inhibition of apoptosis and regulation of cell cycle, is closely related with tumor chemotherapy resistance. The subject liposome-mediated survivin antisense oligonucleotide (antisense oligodeoxynucleotide, ASODN) study to enhance the role of doxorubicin (adriamycin, ADM)-induced apoptosis of hepatoma cells. Methods: by liposome transfection Survivin ASODN into liver cancer cells, using RT-PCR and Western blot Survivin changes MTT colorimetric assay of cell proliferation inhibition was detected by flow cytometry (flow cytometry, FCM) hepatoma cells The rate of apoptosis. Use the ADM after treatment, MTT colorimetric detection of cell proliferation inhibition FCM to detect cell apoptosis index changes. Results: After transfection Survivin ASODN 24 hours in each group, ASODN Survivin mRNA and protein downregulation, the most obvious 800ng/ml group compared with the control group, a significant difference (P lt; 0.05); MTT Survivin ASODN treated cells in each group were decreased proliferative activity; the FCM detected in HepG2 cells 400ng/ml, 600ng/ml, 800ng/ml group of ASODN for 24 hours after the apoptosis rate were 7.45 ± 0.14 %, 13.15 ± 0.37%, 35.75 ± 0.68%, 0.54 ± 0.05% and SODN transfected with control blank group, 0.56 ± 0.08% compared to a significant difference (P lt; 0.05). Plus ADM the 600ng/mlASODN group apoptosis rate of up to 41.35 ± 0.68%, with separate ADM and transfected the ASODN after the apoptosis rate of 16.94 ± 0.54% and 15.07 ± 0.35%, there are significant differences. MTT colorimetric experiments show blank control group with the ADM group and ASODN the group transfected cells 24 hours after the inhibition of cell proliferation significantly different (P lt; 0.05) ADM ASODN group with the other three groups, there were significant sex difference (P lt; 0.05). Conclusion: The liposome-mediated transfection for Survivin design in ASODN Survivin gene can be closed down Survivin protein and mRNA expression and promote apoptosis of HepG2 cells, and inhibit the growth of liver cancer cells. The ASODN closed Survivin expression can enhance the effect of ADM-induced apoptosis of hepatoma cells, presumably increase the ADM chemotherapy sensitivity, improved conventional chemotherapy, with potential clinical value.

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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