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Expression of MCM5 and Its Significance in Cervical Pathological Changes

Author: LiZuo
Tutor: TaoGuangShi
School: Central South University
Course: Obstetrics and Gynaecology
Keywords: Minichromosome maintenance protein 5 Labeling index Chronic cervicitis Cervical intraepithelial neoplasia (CIN) Cervical squamous cell carcinoma
CLC: R737.33
Type: Master's thesis
Year: 2010
Downloads: 14
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Abstract

Background: minichromosome maintenance protein (miniehromosome maintenance-proteins, MCMs) is a group of closely related proteins and DNA replication. Protein family consists of six members, namely MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and of MCM7 (Cdc47). Minichromosome maintenance protein is copied to permit an important factor (replication licensing factor, RLF) members, starting the beginning of DNA replication with chromatin before starting combination marked a copy. MCM proteins exist only in the nucleus of the cell cycle, the mRNA level of MCM proteins in normal cells change with the change of the cell cycle, MCM protein expression at the G1 / s conversion reached a peak when the cells enter the G0 phase and differentiation, aging MCM protein expression levels may not even be able to detect. MCM proteins are expressed at high levels in proliferating cells, quiescent cells or differentiated cells do not express, or very low levels. MCM protein and mRNA expression levels of the organization, is proportional to the degree of cell proliferation, suggesting that MCM proteins can be used as specific markers of proliferating cells. Tumor cells compared to the normal tissue cells in addition to the morphological features on the functional state of having a rapidly proliferating ability, so MCM proteins can be used for cancer research. The MCM5 Is one of the six members of the MCM proteins, detection MCM5 can direct response to the levels of MCM proteins. MCM5 cervix, esophagus, bladder cancer research: MCM5 some tumor cells can be used as markers and diagnostic tools. MCM5 in cervical tissue of chronic inflammation and intraepithelial lesions and cervical squamous cell carcinoma and its significance no system at home and abroad reported. Objective: To investigate minichromosome maintenance protein 5 (MCM5) expression in cervical squamous cell carcinoma and cervical intraepithelial neoplasia (CIN) and its clinical significance. Method: using immunohistochemical methods, respectively, to detect the MCM5 in 30 cases of cervical chronic inflammation of the tissue and 90 cases of epithelial tumor-like lesions (CIN Ⅰ specimens 30 Li, CIN Ⅱ specimens of 30 cases of CIN Ⅲ specimens 30 Li) and 66 cases of cervical squamous cell carcinoma MCM5 The expression analyzed by calculating the labeling index (Labeling index, L Ⅰ) MCM5 relationship between the expression of all levels of cervical lesions and cervical squamous cell carcinoma MCM5 expression and clinical pathological features. All data SPSS13.0 statistical analysis software. Between groups was used to compare the groups rank sum test (Kruskal-Wallis test) was used to compare two independent samples rank sum test (Wilcoxon rank sum test) between the two groups, P lt; 0.05 as the test standard. Results: of MCM5 in the expression of positive signal in the nucleus, cytoplasm, no coloring. In the chronic inflammation of the cervix tissue, only the substrate and the substrate 1 to 2 layers above the parabasal visible positive cells. Varying degrees of dysplasia, shift the number of layers ranging positive cells. Invasive positive cells was significantly increased its distribution to lose the law, mostly diffuse distribution in all epithelial layers and interstitial infiltration. MCM5 protein expression in different cervical lesions to invasive carcinoma from chronic inflammation: of MCM5 in protein expression was progressively increased. The the Chronic Cervicitis group, CIN Ⅰ group, CIN I, CIN III and cervical squamous cell carcinoma, and compared between groups, the differences were significant. In the chronic cervicitis organization, of MCM5 in expression limited to 1/3 to 1/2 of the cells in the gland at the base of the labeling index (L Ⅰ) ranging from 0 to 10.12%, with a median of 5.05%. CIN Ⅰ labeling index (L Ⅰ) ranging from 20.14% to 50.55%, with a median of 36.45%. CIN Ⅱ their labeling index (L Ⅰ) ranging from 41.11% to 74.56%, with a median of 60.43%. CIN Ⅲ its labeling index (L Ⅰ) ranging from 64.78% to 85.21%, with a median of 79.52%. Of MCM5 in the expression and distribution of cervical squamous cell carcinoma, also seen a large number of cells near the surface expression of the labeling index (L Ⅰ) ranging from 76.11% -94.89%, with a median of 90.34%. Normal tissue of the cervix and cervical squamous cell carcinoma the MCM5 LI differences statistically significant P (lt; 0.01). Statistical analysis showed MCM5 expression in cervical squamous cell cervical squamous differentiation degree significant correlation (P lt; 0.01) to express the higher differentiation lower FIGO clinical stage, patient age and lymph node metastasis without significant correlation P (GT; 0.05). Conclusion: 1. Cervical tissue minichromosome maintenance protein 5 (MCM5) is a reliable marker of cell proliferation of MCM5 in the over-expression of the protein is a sign of precancerous lesions. 2 according to the expression of MCM5 staining distribution and labeling index helps distinguish the tissue of the cervix chronic inflammation of the tissue and cervical intraepithelial neoplasia (CIN) and cervical squamous cell carcinoma of MCM5 in protein expression can be used to reference indicators C1N its cancerous. 3.MCM5 expression of the degree of differentiation of cervical squamous cell carcinoma can indirectly reflect the degree of malignancy of cervical squamous cell carcinoma.

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