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L. bicolor leaf salt glands and salt secretion mechanism of

Author: HanJunLi
Tutor: ZhaoKeFu
School: Shandong Normal University
Course: Botany
Keywords: Limonium Salt secretion mechanism Salt glands Ion content Cell vacuole Salt treatment Blade ATPase activity Goblet cells Cytochemical localization
CLC: Q945.78
Type: Master's thesis
Year: 2001
Downloads: 190
Quote: 8
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Abstract


With 100 mmol / L NaCl, NaBr, NaI processing plants, and then were measured leaf secretion amount of Na Br - The results showed that the inhibition of Na secretion, Cl - to promote Na secretion. With 100 mmol / L NaCl, the NaHCO 3 , the Na 2 SO 4 and processing plants, and then were measured blade secretion Na 4 2 - and HCO of 3 - inhibited the Na secretion, promote secretion of Na following CL - . With 100mmol/LNaCl, KCl, CaCl 2 , MgCl 2 , respectively, processing plants, and then the amount of determination of blade secretion cation results show that the salt glands secrete Ca < / sup>, the highest, followed by the Na , the Mg 2 , the K . Cytochemical localization of ATPase activity of lead phosphate precipitation of salt treatment and control L. bicolor leaf salt gland cells, the results were compared with the plasma membrane of the salt treatment salt gland cells were collected and penetrate the area of ??Chinese and foreign goblet cell plasma membrane strong ATPase activity, and control of salt gland cells almost no ATPase activity. This indicates that the ions in the salt secretion process by collecting cell plasma membrane H -ATPase role the initiative to enter the cells were collected ions reach the permeable zone, the goblet cell plasma membrane H outside -ATPase role actively transported into the internal salt gland cells. Salt salt gland cell vacuole membrane with only weak ATPase activity, suggesting that the ions into the vacuole may be a passive process. Ion content in the salt treatment and control Limonium leaves salt gland cells were determined using X-ray micro-analysis methods, NaCl treatment of salt gland cells vacuolar Na , Cl ion content are higher than the cytoplasmic Na , Cl - ion content, which indicates that small vacuoles play an important role in the secretion process. Secreting cells vacuolar Na were significantly higher than adjacent cells within the goblet cells outside the goblet cells, Cl - content is also high, indicating that the salt gland secretory activity not a continuous process, but a recurring event. In the secretory cells, not only in the vacuole ion content is higher, and cytoplasm ion content is higher, indicating that the salt glands secreting cells cytoplasm of salt ions adaptable. Figure it out through the plasma membrane H -ATPase role plants too much salt content is higher, the cells were collected vacuolar Na , Cl - ion collector, and then brought into the internal salt gland cells. Ion content in the vacuole and cytoplasm of epidermal cells and mesophyll cells were significantly lower than the salt gland cells, which indicates that L. bicolor by salt gland activity, effectively reducing the salt content of other cells to maintain their normal physiological functions. Salt treatment the the Limonium cells in K levels were reduced, which is the result of ion balance.

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