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The Effect of Altered Lighting Hours on Depression-Related Behaviors in Rats

Author: WangLiNa
Tutor: FengPingFu;ZhangZuo
School: Zhengzhou University
Course: Neurobiology
Keywords: Illumination Forced swimming test Y - maze test Depression pERK Implicit calcium
CLC: R749.4
Type: Master's thesis
Year: 2009
Downloads: 32
Quote: 0
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Abstract


Depression is a serious impact on the physical and mental health, causing huge loss to society mental disorders. However, the pathogenesis of depression remains unclear, antidepressant treatment has no substantial long-term efficacy. The statistical data to show that the social activities, including the reduction of the sport is one of the important risk factors for depression. A large number of facts show that depression in patients with 5-HT neuronal function. Most antidepressants antidepressant effect is achieved through inhibition of 5-HT re-uptake or inhibiting its degrading enzyme (e.g., a monoamine oxidase inhibitor) to extend the function of 5-HT. Recently found that the the subcellular delivery system neurons play an important role in depression. Neurons subcellular information delivery system including brain-derived neurotrophic factor (brain-derived neurotrophic factor, BDNF), promote the mitogen-activated protein kinase (mitogen-activated protein kinase, MAPK) signaling system, the calcium signal delivery system neurogenesis plays a very important role in the incidence of depression and rehabilitation. MAPK signal transduction system defects may be extreme emotional disorders such as manic depression and endogenous depression a molecular biological markers (Biomarker). In the frontal cortex of suicide in depression (frontal cortex, FC) and hippocampus (hippocampus, HIP) were found in this signaling system in one of the most common protein in the extracellular signal-regulated kinase (extracellular signal-regulated kinase, ERK) phosphorylation reduce . This change in the rat model of depression, has been confirmed. In addition, we found in preliminary experiments, continuous illumination inhibit rat voluntary movement. The increase in long-voluntary movement can increase brain implicit Calcium 2 (calsequestrin2, CASQ2) content, improve depression. ERK phosphorylation levels will change is not known. Therefore, the present study was to investigate the change the day light time on a variety of behavioral and brain ERK phosphorylation (extracellular signal-regulated kinasephosphorylation, pERK), and the level of CASQ2. Objective: To implement the growth and development of the rat is not the long light, the observed behavior in the forced swim test (forcedswimming test, FST) and the Y-maze test (Y-maze test) change, and to detect brain pERK The CASQ2 protein level changes, to analyze the light to change the mood, and to explore the possible mechanism of the light in antidepressant. Method: an animal preparation: on 5wSD rat implement not drive light treatment (control group, the group of short-time illumination, long light group, a short light recovery group, long light recovery group) 8w, short-time light group and long When lighting group in dealing with the end of instantly behavioral experiment, short light recovery recovery the 1w re experimental group and the long light recovery group processing. Behavioral tests: 5min behavior in the rat recorded in the FST, analysis of its climbing, swimming and immobility time, to determine the degree of mental depression; recorded in the Y-maze test rats in learning and memory. the frequency and duration of the kinds of judgments, and the determination of the ability of learning and memory. 3.pERK and CASQ2 protein expression levels: the FST and Y-maze test after 2d, were decapitated, all rats, the hippocampus (hippocampus, HIP), prefrontal cortex (prefrontal cortex, PFC), hypothalamus (hypothalamus HT) and frontal cortex (frontal cortex, FC) organization, extracted brain tissue protein, Western blot analysis of various brain regions pERK CASQ2 content. All experiments were carried out between 8am-12am. Statistical analysis: one-way ANOVA mean differences between each group were compared between the groups using the chi-square test was used to compare the percentage differences. Results: 1. Forced swim test results in each experimental group of rats in the FST sports (climbing swimming) and fixed time with the control group of rats were significant differences compared and the differences were statistically significant. Wherein the short illumination rats compared with control group, regardless of light before and after recovery, its movement (Climbing swimming) times are extended, both shortened the immobility time; light before and after recovery of the long light group rats and control group compared to rats with short-time illumination opposite. The 2.Y-maze experimental results of experimental rats in the Y-maze learning and memory performance and control rats compared to the differences were statistically significant. Short time illumination rats compared with the control group, regardless of light before and after recovery, the total number of learning and memory, the number of errors and the total time spent significantly reduced; rather light before and after the restoration of the long light rats Contrary to rats with short-time light group compared with the control group. Experimental results 3.pERK and CASQ2 protein expression levels compared with the control group, the experimental group, HIP, PFC, HT and FC 4 brain regions pERK1 / 2 protein expression levels were different, but this difference was not statistically significance. Short time illumination rats compared with the control group, regardless of light before and after recovery, Part 4 brain regions pERK1 / 2 protein expression levels were increased; rather light before and after the restoration of the long light of rats with control group compared to rats with short-time illumination opposite. However, the short-time illumination rats with long light rats, Part 4 brain regions pERK1 / 2 protein expression levels were significantly increased, and the difference was statistically significant (P <0.05). CASQ2 protein expression levels were compared before and after each experimental group rats HIP, PFC, HT and FC 4 brain regions in light recovery differences were statistically significant (P <0.001). Short time illumination rats compared with the control group, regardless of light before and after recovery, Part 4 brain regions CASQ2 protein expression were significantly increased; rather long when the light before and after the restoration of the light group of rats with the control group compared to opposite rats with short-term light group. Conclusion: 1. Illumination time change the behavior of rats in the FST in the Y-maze learning cognitive ability and brain pERK1 / 2 corresponding changes light change is one of the important factors that affect rats emotions. Change the illumination time the rat brain CASQ2 level change accordingly, indicating that the light change is one of the important factors affecting the spontaneous movement. Developmental stages in the growing season, to give the light of the process of the adult rat depression-related behavior and brain substance will not impact the normal light of the short-term recovery. Changing the exposure time may be by changing the movement to affect rats emotions.

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