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The Expression and Srginficiance of the Tissue Inhibitor of C-FOS、MMP-9 and TIMP-1 in Endometriosis

Author: LiuHanSheng
Tutor: YuXinYan
School: Taishan Medical College
Course: Obstetrics and Gynaecology
Keywords: c-fos MMP-9 TIMP-1 Endometriosis Eutopic Matrix degradation
CLC: R711.71
Type: Master's thesis
Year: 2010
Downloads: 45
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Abstract

Objective To detect the proto-oncogene c-fos, matrix metalloproteinase -9 (Matrixmetalloproteinase-9, MMP-9) and tissue inhibitor of matrix metalloproteinase -1 (Tissue inhibitors of metalloproteinase-1, TIMP-1) in normal subjects and uterine endometriosis (endometriosis, EMT) in patients with endometrial carcinoma, analyzing the correlation between the three explore the c-fos, MMP-9 and TIMP-1 in the development of the role of EMT and is EMT treatment provide a theoretical basis. Methods EMT eutopic endometrium specimens (n ??= 27) and ectopic endometrial samples (n = 27) and normal endometrium specimens (n ??= 26). By immunohistochemistry SP method were used to detect c-fos, MMP-9 and TIMP-1 in the above three groups of cells, the expression level. 1, in normal endometrium, EMT eutopic and ectopic endometrium can be detected in c-fos, MMP-9 and TIMP-1 expression. c-fos protein staining mainly distributed in endometrial epithelial cells and stromal cell nuclei, MMP-9 and TIMP-1 is mainly distributed in endometrial epithelial and stromal cells in the cytoplasm. 2, compared with normal endometrium, c-fos in eutopic and ectopic endometrium EMT expression levels increased, the difference was statistically significant (Hc = 11.9970, P = 0.0025); while it is in the patient EMT between eutopic and ectopic endometrium expression levels were not significantly different; endometrium in three groups, c-fos protein expression levels were significantly higher in the proliferative phase secretory phase p lt; 0.05). 3, MMP-9 in patients with ectopic endometrium EMT expression was significantly higher than in normal endometrium and EMT eutopic endometrium (P lt; 0.05), the EMT expression in eutopic endometrium and normal endometrium difference There was statistically significant (P lt; 0.05), in the normal endometrium and eutopic endometrium showed a menstrual cycle-dependent expression, expression intensity than proliferative phase secretory phase (P lt; 0.05); EMT ectopic No cyclical endometrial expression intensity difference (P gt; 0.05). 4, TIMP-1 expression in EMT ectopic endometrium and eutopic endometrium was significantly lower than normal endometrium (p lt; 0.05), in the normal endometrium was significantly higher than EMT eutopic endometrium, the difference There was statistically significant (P lt; 0.05). In normal endometrial tissue in the expression of TIMP-1 showed cyclical changes in the proliferative phase was significantly higher than the secretory phase (P lt; 0.05); while patients in EMT eutopic and ectopic endometrial tissue lost cycle sex change, proliferative and secretory expression showed no significant difference (P gt; 0.05). 5, in the three groups endometrium c-fos and MMP-9 expression in a positive correlation (P lt; 0.05), the two are synergistic. 6, in the normal endometrium of MMP-9 and TIMP-1 is a negative correlation between the expression (rs = -0.580, P lt; 0.05), both mutual restraint, while in the EMT eutopic and ectopic endometrium changes in both the organization does not have a correlation (rs = 0.385, P gt; 0.05). Conclusions and significance in normal endometrium, EMT eutopic and ectopic endometrium in c-fos, MMP-9, and TIMP-1 were expressed. And c-fos in the three groups cyclical endometrial expression may be related to estrogen and progesterone regulation. EMT ectopic tissue c-fos, MMP-9 and the high expression of TIMP-1 expression in low formation and progression of EMT has a very crucial role. c-fos, MMP-9 and TIMP-1 through interaction ability to enhance the degradation of the surrounding matrix, endometrial and easy to enhance the viability of the reign of endometrial shedding, which is possible with the menstrual blood reflux into the abdominal cavity to survive, and planted in the uterine cavity and infiltration of the surrounding tissue. EMT eutopic and ectopic endometrium in c-fos expression and MMP-9 was a positive correlation between the two collaborate to promote the formation and progress of EMT heavier, while ectopic endometrium of MMP-9 and TIMP-1 between no correlation between the two imbalance, and this has nothing to do with the menstrual cycle variation. Dynamic equilibrium is broken also contributed an important factor in the development of EMT. Tips for eutopic therapy may become a source of new research directions EMT treatment, and c-fos, MMP-9 and TIMP-1 may become a source of sub-EMT important target for therapy.

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CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Other diseases of the female genital > Endometriosis
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