Dissertation > Excellent graduate degree dissertation topics show

Association Analyses of SUR1 Gene and KCNJ11 Gene Polymorphisms with Gestational Diabetes Mellitus in Guang Dong Hans in China

Author: FengQing
Tutor: LiuChao
School: Guangzhou Medical College
Course: Biochemistry and Molecular Biology
Keywords: Gestational Diabetes Mellitus SUR1 gene KCNJ11 gene DNA polymorphism
CLC: R714.256
Type: Master's thesis
Year: 2010
Downloads: 23
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveGestational Diabates Mellitus (GDM)is a metabolic disease with complex genetic and environmental components.It has many genetic and environmental components similar to type 2 diabetes.We investigate the allelic frequency of Sulfonylurea receptor 1 gene ( SUR1 ) and Kalium inwardly recitifier gene (KCNJ11)of gestational diabetes mellitus (GDM) women to find out the susceptible gene associated with GDM and the relationship between these genes and the clinical characteristics.MethodsFifty-three GDM women were served as study group, fifty normal pregnant women were selected as control group.The distribution of SUR1 rs1799854、rs1799859 and KCNJ11 rs5219 alleles frequencies of these pregnant women were examed by polymerase chain reaction(PCR)-restriction fragment length polymorphism (RFLP) method combined with direct sequencing. Height、weight、blood pressure and fasting blood glucose were measuered in all subjects.Independent sample t test was used to compare means between two groups.ANOVA was employed to compare the differences of clinical characteristics between genotypes.Chi-square test was employed to test the the differences of genotypic and allelic frequencies between cases and controls.Association analyses were done by logistic regression.All statistical analyses were calculated by the software of SPSS 12.0 for windows. Results:1.There was significantly difference of the frequency of genotype GA (rs1799859) between GDM group and control group (32.1% vs 8.0%,p=0.002). Also, there was significantly increased alleles frequency of A(rs1799859) between GDM group and control group. (16% vs 4%,p=0.004).2.There was significantly difference of the frequency of genotype EK、KK(rs5219) between GDM group and control group (71.1% vs 14.0%,20.8% vs 14.0%,p=0.038).3. Results from multiple logistic regression analysis showed that the A - G transition polymorphism of SUR1 rs1799859 (OR = 6.82,95 %CI 1.90～24.54) and the A - G transition polymorphism of KCNJ11 rs5219 ( OR = 3.90, 95 %CI 1.02～15.00) were associated with the development of GDM.4.The association between SUR1 rs1799854 and the development of GDM was not found.Conclusions:The SUR1 gene 31 exon polymorphism (rs1799859) and the KCNJ11 gene E23K polymorphism (rs5219) are associated with GDM in Han Chinese in Guang Dong. These polymorphism may have a diabetogenic effect by changing the function of ATP-sensitive KATP channels, which impair glucose-induced insulin release of the health and make pancreaticβ-cell oversensitively. The SUR1 gene 16 exon polymorphism (rs1799854) is not associated with GDM in Han Chinese in Guang Dong.

Related Dissertations

  1. Research of Susceptibility Genes of Pediatric Diabetes Mellitus,R725.8
  2. Analysis of Related Factors and Outcomes of Gestational Diabetes Mellitus between Different Nationalities in Xinjiang,R714.256
  3. Silkworm genome of dinucleotide SSR of trinucleotide SSR polymorphism,S881.26
  4. Association Analyses of KCNJ11 and SUR1 Gene Polymorphisms with Type 2 Diabetes in Han Chinese in Hubei,R587.1
  5. Cardiomyocyte Apoptosis and Genomic Polymorphisms in Sinoaortic-denervated Rats,R965
  6. Chemical Substances Change and Molecular Mechanism of the Wheat Blossom Midge, Sitodiplosis Mosellana (Diptera: Cecidomyiidae) during Diapause,S435.122.1
  7. Study on the Differentially Expressed Genes in Peripheral Leucocytes and Placenta Tissue of Gestational Diabetes Mellitus Patients,R714.256
  8. Molecular Cloning, Classification and Development of Molecular Markers of Wheat LMW-GS Genes,Q943.2
  9. Study on the Mechanism of Insulin Resistance Induced by Tumor Necrosis Factor-α and Soluble Receptor and Gene Polymorphism in Gestational Diabetes Mellitus,R714.256
  10. Analyses of Molecular Genetic Polymorphisms of the H-FABP Gene and Their Associations with Intramuscular Fat Content in Pigs,S828.2
  11. Genetic Heterogeneity and Gene Diagnosis of Autosomal Dominant Polycystic Kidney Disease Using Microsatellite DNA and Single Nucleotide Polymorphisms,R692.1
  12. Studies on the Transformation of Genomic DNAs of Distant Resources in Rice,S511
  13. Comparative Studies on Schisandra Chinensis and Schisandra Sphenanthera,R282
  14. Studies on the Molecular Taxonomy and Phylogenetic Relationships Within the Genus Pleurotus,Q943
  15. Studies on Cloning of Chicken Fatty Acid Binding Protein Genes and Their Relationships with Intramuscular Fat Content,S831
  16. Development and Characterization of Microsatellites in Cultivated Peanut (Arachis Hypogaea L),S565.2
  17. The Chinese narcissus the cobalt -60Gamma ray irradiation mutagenic effect and mechanism study,S682.21
  18. The Relationship between Obesity and Adverse Pregnancy Outcomes and Its Mechanisms Involved,R714.2
  19. Studies on Biological Characters and Inheritance of Fusarium Moniliforme Sheld,S432.44
  20. Establishment of Macaca Monkey Heredity Monitoring Method and Analyses of Genetic Diversity by Microsatellite DNA Markers,S865.16

CLC: > Medicine, health > Obstetrics and Gynaecology > Obstetrics > Pathological pregnancy ( abnormal pregnancy ) > Complications of pregnancy > Polyendocrine and metabolic diseases
© 2012 www.DissertationTopic.Net  Mobile