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Objective: Type 2 diabetes is a common metabolic diseases with complex genetic background. Because of its high degree of genetic heterogeneity between different races of international reports on type 2 diabetes susceptibility region and candidate gene there are big differences. The selected encoding islet β-cell K of ATP channel two subunits KCNJ11 gene and SUR1 gene as a candidate gene to investigate the KCNJ11 gene E23K polymorphism and the SUR1 gene 16 exon-3t / The correlation c polymorphism and Hubei Han population with type 2 diabetes. Methods: compatriots internal control (pedigree) and random cases - control two experimental design. Using polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis of 400 specimens of Hubei Province KCNJ11 gene E23K polymorphism, 125 cases, controls 275. By PCR-RFLP analysis of 464 cases of samples SUR1 gene 16-3t / c polymorphism 306 cases against 158 ??people, including 70 type 2 diabetic sibling pairs. All subjects were physiological and biochemical indices, height, weight, waist circumference, hip circumference, blood pressure and fasting blood glucose was measured to calculate body mass index (BMI) and waist-to-hip ratio (WHR). All samples were divided into three groups for analysis: All cases and controls, type 2 diabetes and normal compatriots as well as random cases unrelated normal controls. Compatriots clinical variables between the two samples of cases and controls mean compared using independent samples t test using a paired t-test, each genotype were compared using one-way ANOVA. Genotype and allele frequencies between the two groups was used to compare the x 2 test, correlation analysis Logistic regression. Statistical analysis using SPSS11.5 statistical software. Results: (1) study the random case - control design, KCNJ11 gene the E23K polymorphism genotype frequency allele frequencies between type 2 diabetes and control groups significant differences (P <0.05 or P < 0.01); Logistic regression analysis showed that: random cases - control Univariate found KCNJ11 gene E23K mutation and type 2 diabetes significantly related (EK EE compared to OR = 2.000, P = 0.028; KK and EE compared to OR = 2.373 , P = 0.004), adjusted multiple factors by age, sex, blood pressure and obesity, there are still significant correlation (EK EE compared to OR = 1.978, P = 0.039; KK EE compared to OR = 2.472, P = 0.004 ); All subjects, whether single factor or adjusted by the multiple factors of age, sex, blood pressure and obesity the KK genotype with type 2 diabetes showed a significant positive correlation (not before adjustments OR = 2.303, P = 0.004; adjustment After OR = 2.341, P = 0.006). (2) three-group experimental design of SUR1 gene 16-3t / c polymorphism genotype and allele frequencies between type 2 diabetes and control groups had no significant difference (P> 0.05), all the sample stratified analysis by gender, age, and BMI levels not observed significant differences. Logistic regression analysis showed that: both single factor or multi-factor adjustment by age, sex, blood pressure and obesity ,16-3t / c polymorphism with type 2 diabetes was no significant correlation. ANOVA analysis showed that, in all subjects and in the case group the SUR1 gene three genotypes between the waist and hip were significantly different. In addition, clinical data comparison between the case and control groups BMI have significant differences, the case group was significantly higher than that of the control group, the single factor logistic regression analysis showed that obesity is an independent risk factor for type 2 diabetes. Conclusion: Han population in Hubei, the development of type 2 diabetes with KCNJ11 gene E23K polymorphism associated, but not significantly correlated with SUR1 gene 16-3t / c polymorphism. The KCNJ11 gene the Hubei Han, a type 2 diabetes susceptibility genes.
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