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With the development of modern biotechnology, microorganisms in environmental protection increasingly broad application prospects. This article on microbes in desulfurization of fossil fuels Strains, strain identification, Staphyloccus sp.SB113 strains microbiology characteristics, Staphyloccus sp.Strain SB113 removal of the fuel organic sulfur conditions optimization, as well as the strain degradation of organic sulfur The mechanism of preliminary research. To thiophene compounds of the organic sulfur mode and the sole source of sulfur, the organic sulfur content changes by comparison before and after fermentation strain selection and screening strain obtained ultraviolet and DES mutagenesis, having higher degrading organic sulfur efficiency mutant was SB113. Identification of the morphological characteristics, physiological and biochemical characteristics of strain SB113 separation and purification, and bacteria, and bacterial growth characteristics, and reference \Staphylococcus, and named Staphyloccus sp.Strain SB113. Degradation of organic sulfur in order to Staphyloccus sp.Strain SB113, the experiment is not only single-factor test strains of seed culture conditions and seed medium formula optimization and single factor experiment and orthogonal experiment combining Staphyloccus sp . Strain SB113 organic sulfur conversion conditions. The experimental results suggest that the beneficial microbial desulfurization of the seed culture conditions are: (1) Culture medium - glucose 2.0%, peptone 1.0%, ammonium chloride 1.0%, thiophene 2.0%, of CaCl the 2 0.001%, ZnCl 2 0.005%, MgCl 2 0.1%, KH 2 PO 4 0.1%; (2) bacteria pH6.8, temperature 36-37 ℃, shaking speed to take the-160rpm conditions, cultured 16h. Strain the Staphyloccus sp.SB113 into organic sulfur diesel optimal conditions: inoculum size of 8.0%, temperature 32 ° C, the strains transformed time 28-32h, the pH value of 6.5-6.7, 180-shaker at 220 rpm. Orthogonal experiment results show that the five factors of temperature, time, initial pH, fermentation inoculum size and shaker speed, the influence of the organic sulfur conversion efficiency of microbial the Staphyloccus sp.Strain SB113 strains for fermentation inoculum size and fermentation time, the shaking speed desulfurization least significant. Conducted a preliminary study, the conclusion is: the strain could be diesel in the water insoluble organic sulfur into soluble organic sulfur, and thus achieve the purpose of desulphurization with fuel separation of Staphyloccus sp.Strain SB113 desulfurization mechanism.
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