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Fine Structure of Pig Skin and Determination of the Damage of Enzyme on It

Author: WangYingMei
Tutor: LiZhiQiang
School: Sichuan University
Course: Leather Chemistry and Engineering
Keywords: Fibrils Fine structure Pigskin
CLC: TS512
Type: Master's thesis
Year: 2002
Downloads: 133
Quote: 1
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Abstract


The organizational structure and its changes in the preparation stage of the tanning of animal skins occupies an important position in the leather of scientific research, engineering value is important for the improvement of the design and control of the tanning process, improve the quality of leather products. In this study, using transmission electron microscopy and scanning probe microscopy methods, combined with computer graphics and image processing technology, in-depth study and characterization of the fine structure of the pig skin tissue fibrils based on changes in the enzyme and destruction. Study and determine the scanning probe microscope natural pigskin and its hydrolyzate observational studies rhyme method, whereby observation to the the pigskin surface of the fibrils 67 nm periodicity of peak-valley structure, and the enzymatic hydrolysis of the single root of the original fibrous structure, and the above structure quantified characterized. And established the original three-dimensional structure of the fibers and adjacent fibrils between the lateral and axial ordered aggregation mode. The point is: the fibril cross section mainly for the circular and elliptical cross-sectional diameter, due to different thickness levels vary, approximately 50 to 150 nm; ends of the original fiber the slightly swollen spherical, stripes cycle disappears; the original fiber length is small than 10 microns, no branch structure; between the fibrils tightly orderly arranged in a crystalline structure, but have different degrees of defect; fibrils exist in the axial direction overlap each other. Pigskin epidermis and the fine structure of the basement membrane and the change in the enzyme. To determine the thickness of the three-layer structure of the basement membrane is 50-100nm; the enzymatic unhairing the Chengzhong Ji film bright band disappeared, if prolonged, granulocytes mask gradually disappear, exposing papillary layer fibril structure. The research identified the pigskin organization Zhongyuan fiber, the variation of the fine structure of the micro-fibrils enzyme and its mechanics made of leather with enzyme depilation process performance. Enzyme linkages between fibrils weakened generate free fibrils; gradually intrusive role in the microfiber surface, eventually leading to the collapse of the crystalline structure of micro-fiber; fibrils little change in the cross-sectional shape, but the shorter length. The results explain a the enzymatic depilation made leather exhibited softness and tensile strength decreased. The research to deepen the understanding of the the pigskin organizations in fibrils level fine structure and its change in the enzyme, the pigskin organizations will provide important theoretical basis as leather or biological materials modification technology.

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CLC: > Industrial Technology > Light industry,handicrafts > Leather Industry > Leather school > Histology of raw hides and skins
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