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Liver regeneration is a complex process involving cell activation, de-differentiation, proliferation and proliferation regulation, differentiation and reconstruction of tissue structure and function of a variety of physiological and biochemical activities. Cellular signal transduction is the control center of life activities, cell growth, proliferation, differentiation, apoptosis, metabolism, and other physiological and biochemical activities are subject to the regulation of signal transduction. A variety of growth factor in rat liver regeneration (Autocrine) through autocrine or paracrine (Paracrine) activate various growth factor signaling pathways involved in liver regeneration. Liver hepatocytes, biliary epithelial cells, hepatic stellate cells, sinusoidal endothelial cells, Kupffer cells, pit cells, dendritic cells and other cells and liver regeneration stays at the tissue level. Hepatic stellate cell growth factor signaling pathway genes with liver regeneration at the transcriptional level, using the two-step perfusion of the portal vein dispersed liver cells, Percoll density gradient centrifugation and immunomagnetic bead separation method of separation of regenerating liver astrocytes regeneration of hepatic stellate cell growth factor signal, after the Rat Genome 230 2.0 microarray the rat 2/3 hepatectomy and operative control in recovery 2,6,12,24,30,36,72,120 and 168 h pathway related gene expression using fluorescence real-time quantitative PCR detection of the reliability of the results of microarray analysis, bioinformatics and systems biology analysis of related gene expression pattern and role in the regeneration of hepatic stellate cells in rats. Fluorescence real-time quantitative PCR testing renewable hepatic stellate cell marker gene expression that chip results with real-time PCR test results are basically the same, reliable microarray results, bioinformatics and systems biology analysis of liver regeneration gene changes and the expression pattern and role that expression in rat regenerating hepatic stellate cells, 38 TGF-β signaling pathway related genes with liver regeneration, liver regeneration, the initiation and progression of stages, TGF-β signaling pathway inhibition of hepatic stellate cell growth and proliferation to the termination phase, inhibition weakened addition, TGF-β signaling pathway through the regulation of hepatic stellate cell differentiation, inflammation, extracellular matrix synthesis and other physiological activities involved in liver regeneration; 68 WNT signaling pathway genes associated with liver regeneration, 38,28 and 5 liver regeneration gene belonging WNT / β-catenin WNT/Ca2 and PCP signaling pathway, the WNT signaling pathway-related genes in the regulation of hepatic stellate cell survival, metabolism, proliferation function and differentiation; 77 signaling pathways involved in the MAPK, PI3K and Ca2 mediated growth factor and its receptor gene in rat liver regeneration, in addition to the growth factor and its receptor gene, 95,82 and 43 a liver regeneration genes belonging to the MAPK signaling pathway of PI3K signaling pathway and Ca2 mediated signaling pathway, these genes through the regulation of hepatic stellate cells inflammatory response, survival, proliferation, differentiation, migration and other physiological activities involved in liver regeneration. Overall, the growth factor signaling pathway gene expression patterns varied, complex and related genes expressed in the start-up phase of liver regeneration starting, more than the total expression tune expression mainly play a role in the whole liver regeneration.
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