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To PCBs exposure to changes in the fine structure of the major organs of the experimental fish (Organization) and several activity

Author: ZhangFengJun
Tutor: ZhengWenBiao；FangZhanQiang；WuYuYuan
School: South China Normal University
Course: Zoology
Keywords: PCBs Zebrafish(Brachydanio rerio) swordtails(Xiphophorus helleri) morphology SOD Na~+/K~+-ATPase toxicity LC50
CLC: Q959.4
Type: Master's thesis
Year: 2002
Downloads: 518
Quote: 10
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Abstract


Polychlorinated Biphenyls (PCBs) are man-made organism. Due to it’s stable quality, not easy to be fired and well insulative, PCBs has been used in industry widely. Now, PCBs contaminated the world badly especially in the water environment. Because PCBs is persistent and lipophilic, it was easily absorbed by creature, and enriched in the fishes by food chain. So, PCBs-exposure ubiquitous make the huge damage to fishes and mankind.Zebrafish (Brachydanio rerio) were exposed to PCBs concentrations of ON 50ug/L for 4 weeks respectively. The gill and the liver morphology were examined by transmission eletron microslogy. Xiphophorus helleri were exposed to PCBs concentrations of (K 2ug/L> 50ug/L for 4 weeks, then revealed the pathological alteration by transmission eletron microslogy and scanning eletron microslogy. The activities of SOD and NaVK+桝TPase in 72 hi of swordtails(A7/?/2op/jorws /!e//en)were measured after the PCBs exposure respectively.The 30-day LCso of grown fish and 96 hr LCso of parr fish were measured after swordla\\s(Xiphophorus helleri)were exposed by PCBs.With regard to zebrafish with PCBs-exposure, morphologicalexamination revealed that the membrane and partial cytoplasm of the epithelium cell in secondary lamella collapsed with lots of mucous and cell debris lying around. And the hepatocytes which exposed to PCBs for 4 weeks showed increase in the number of secondary lysosome, the phagocytosis of kupffer cell heightened.The distinct pathological alteration occurred to swordtai\s(Xiphophorus helleri) which exposed to PCBs. Compared to the control groups, in the hepatocytes of exposed fishes, there were lots of lipid drops dispersive and lots of lipofuscin granule, on the same time, rough-surfaced endoplasmic reticulum hyperplasy abnormally, and some mitocondrium collapsed There were distinct alteration in gill filament and gill lamellae, the secondary lamella atrophy, and the supporting cells showed there were more secretion in cytoplasm.appeared abnormal, the abnormal nuclear also was found. The second lamellar and hepatocytes examined using scanning eletron microslogy revealed the second lamellar have damnification and canker. Because of the shrink of the secondary lamellar, the distance between two secondary lamellar broden. In the surface of liver, some injured hepatocyte can be found.In this study, the result indicated that the activities of SOD in liver and ovary were influnced by PCBs remarkblely. There were a raise trend in the first 12 hr and then there are a down trend coupled with the extend of exposure time. However, the gill SOD activity was seldom be induced by PCBs. The gill Na+/K+-ATPase was inhibited by PCBs remarkably in PCBs-exposed groups. But the inhibitions to hepatic Na+/K"-ATPase and ovary Na+/K+-ATPase were not so remarkably.56When the swordtails (Xiphophorus helleri)exposed to PCBs, the 30-day LC50 of grown fish is 10.580ng/L and the 96 hr LCso of parr fish is 2.063ng/L.

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CLC: > Biological Sciences > Zoology > Animal taxonomy (system zoology ) > Vertebrate > Pisces
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