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[Objective] pleural effusion exfoliated cells nucleus DNA content was measured by flow cytometry technology, and pathology of this method with the traditional method, the detection of tumor markers CEA compared to identify pleural effusion, benign and malignant. [Method] specimens from fresh specimens of pleural effusion, heparin, stained with fluorescent dye, flow cytometry nuclear DNA ploidy analysis of cell proliferation, in order to detect CEA, and exfoliated cells pathological examination of statistical methods to test results. [Results] According to the clinical results of the pleural effusion is divided into benign and malignant groups in malignant pleural effusion group, 87.5% of the specimens can be detected by DNA aneuploidy, independent aneuploid peaks in different times the peak of the specimens, two cases of SPF and PI values ??can also be sentenced for the FCM positive, its sensitivity to as high as 95.8%, and CEA sensitivity was 76%, but no significant difference between the two ( p> 0.05). Off cytopathology examination only 8 cases were positive, the sensitivity of only 32%, compared with the first two methods, the difference was significant (p <0.05). In the benign group, 75% of cases showed diploid, and false-positive specimens, two cases of empyema, one case was transudate easy identification of malignant pleural effusion, pleural fluid routine examination can be removed, and its specificity up to 90%, the false-positive cases, by the SPF and PI values ??that the proliferative capacity of the cells is not high. CEA determination in the benign group no false positive, and a specificity of 100%, specificity of exfoliated cells pathological examination is 100%. FCM DNA determination makes the pathological diagnosis can be based on objective indicators, is a useful supplement for pathological examination, and CEA determination of its higher sensitivity compared to somewhat less specificity combined determination can improve malignant pleural plot liquid flow cytometry DNA ploidy determination in the differential diagnosis of benign and malignant pleural effusion diagnostic value of Chinese Abstract accuracy and diagnostic yield. [Conclusion] flow cytometry can quickly detected large numbers specimens, the experimental method is relatively simple, the experimental results of the determination can be based on objective indicators, and the results are high sensitivity and specificity in the differential diagnosis of pleural effusion good , malignant on clinical significance, is the complement of the pathological examination, together with the tumor marker detection can further improve the diagnostic yield.
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