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Objective: To investigate the impact of the five blood circulation parties myocardial apoptosis of acute myocardial ischemia (heart blood stasis model) and mechanisms, relatively parties Differential role. Method: press the weight layered, then the random number table random 35 dogs were divided into seven groups: control group, myocardial ischemia group, Salvia drink group, blood Xuefuzhuyutang group, Shixiaosan group, reviving the spirit efficiency Dan groups pink Siwutang group, administered orally once daily dose of 20ml/kg, the control group, and myocardial ischemia group to distilled water, ig 5 days. Each dose group in the five days after administration threading ligation of the left anterior descending artery at the second diagonal branch, resulting in acute experimental model of myocardial ischemia (heart blood stasis model), and the control group, only threading without ligation. Modeling after 3h blood, serum creatine phosphate kinase (CPK), lactate dehydrogenase (LDH). Heart tissue after the animals were killed after the end of the experiment, the removal of the animal heart drying, weighed, determination of malondialdehyde (MDA) and superoxide dismutase (SOD), the myocardial histopathology pathological changes in cardiac myocyte apoptosis and related protein Bc1-2, Bax expression. Results: 1. Blood Xuefuzhuyutang active effective remedy to cure. The soup of Taohongsiwu, Salvia drink can effectively alleviate the damage caused by ischemia on myocardial enzyme leakage reduction, CPK, LDH levels were lower than the ischemia group among the four groups, no significant differences. Myocardial ischemia group and Shixiaosan group CPK of LDH was significantly higher in the control group showed significant Shixiaosan can not effectively reduce the leakage of the enzyme. 2. The blood Xuefuzhuyutang active effective remedy to cure, pink Siwutang, Salvia drink can significantly improve ischemic myocardial tissue SOD activity, decreased MDA content at the same time, in order to mitigate the damage of lipid peroxide was no significant difference among the four groups . Shixiaosan not effectively alleviate the damage of lipid peroxidation. 3. No significant cell degeneration and necrosis in the control group, only to see a very small amount of apoptotic cells; myocardial ischemia group Shixiaosan group significantly increased cardiomyocyte apoptosis, myocardial necrosis; Salvia drink group, Xuefuzhuyu, decoction group, active spiritual effect Dan group the soup group myocardial Taohongsiwu apoptosis and necrosis were significantly reduced. 4. Myocardial ischemia group Shixiaosan set of apoptosis-related protein Bax was significantly increased Bcl-2 expression was significantly reduced; Salvia drinking group, Xuefu gradually the scabies soup group, active effective remedy to cure the group, pink the Siwutang group with myocardial ischemia the blood group and Shixiaosan of group,: Run apoptosis related proteins Bax expression was significantly decreased Bcl-2 expression increased significantly, there are significant differences among the four groups showed no significant differences. Conclusion: Xuefu gradually addiction soup active efficiency panacea, pink Siwutang, Salvia drink effectively the leakage of the enzyme reduced significantly improve ischemia. Muscle tissue the SOD, tongue, decreased MDA content at the same time, in order to reduce the damage of lipid peroxidation, effectively promote the expression of Bcl-2 related proteins spoon, inhibit the expression of related proteins Bax, thereby effectively inhibit myocardial apoptosis and necrosis, reduce the myocardial cells injury, has a protective effect on myocardial ischemia. The acute shortage Shixiaosan as. Muscle no significant protective effect, which can be Meng the with Shixiaosan (((Taiping Benevolent Dispensary Bureau))) Prescription characteristics. The the prescription Wulingzhi and Puhuang good hepatic blood points to Tom Lee the blood scattered addiction the blood but lack the power of the gas line. Xuefu-by-addiction soup, active effect a panacea, pink Siwutang, the party stressed qi and activating blood and Salvia drink four groups, so that the gas line is the blood line. Both xue min addiction stagnation, and vent points stagnation, and use it to make the addiction to the gas line. Xuefu gradually scabies soup, to active effective remedy to cure, Taohongsiwu stirred up, Salvia drink can be more effective inhibition of cardiomyocyte apoptosis and necrosis, reduce myocardial cell injury. At the same time, according to the results of this study can be inferred Xuefu-by scabies soup active efficiency panacea, pink Siwutang, Salvia drink four square treatment effort transvestite certificate has been able to effectively inhibit the occurrence and development of ischemic myocardial apoptosis this quartet can effectively suppress the leakage of enzymes to reduce the damage of lipid peroxides, scavenging free radicals, promote apoptosis-related protein Bcl-2 expression and inhibition of protein B expression. So as to achieve the protective effect of ischemic myocardium, reduce myocardial apoptosis. The experiment by observing the differences between the parties role, initially to clarify the mechanism the five blood of the addiction side of the acute ischemia in dogs (effort addiction syndrome model) lining muscle apoptosis intervention role, prompted in healing and formulas targeted for medicines further in-depth research and clinical applications to provide experimental evidence. -
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