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Strain identification and genotyping of Mycobacterium

Author: LiuGuan
Tutor: ZhaoYanLin
School: Beijing Tuberculosis and Thoracic Tumor Research Institute
Course: Internal Medicine
Keywords: Mycobacterium Identification of bacteria Mycolic acid Genotypic analysis ECL VNTR Resistance Typing methods Mycobacterium Isolates mycobacteria Molecular Epidemiology tuberculosis TB patients Resistance rates Composite group Strain identification Genus Sequencing analysis Bacterial
CLC: R52
Type: Master's thesis
Year: 2010
Downloads: 114
Quote: 0
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Abstract


Fast strain identification has an important role in the early diagnosis and timely and appropriate treatment of the underlying disease, early detection and treatment of infectious diseases is the premise of early control its spread. Research progress based on the identification of Mycobacterium' dissertation">Mycobacterium genotyping aspects of the subject to be adopted from the bacteria levels and genotype level research Mycobacterium classification for clinical fast, accurate identification of mycobacterium species and analysis of TB prevalence provide a reference. Strain identification of Mycobacterium this topic mycolic acid sequencing method jointly identified by HPLC analysis of 118 clinical isolates of Mycobacterium nitrobenzoic acid medium screening, found that 11 sequencing identification method results mycolic acid bacteria Mycobacterium tuberculosis complex identification results inconsistent nitrobenzoic acid, high pressure liquid mycolic acid analysis identification and DNA sequencing compliance rate of 91.53%, including tuberculosis sub- sticks tuberculosis complex identified in line with the rate of 94.59%, 90.12% non-tuberculous mycobacteria. Concluded that mycolic acid clinical isolates of Mycobacterium strain identification is a fast, easy and accurate method of disposal emergency and public health emergencies in the identification of Mycobacterium certain Mycobacterium multiphase identify important part of strategy can be used as reference value. In the area of ??molecular epidemiology, the subject application 24 points MIRU typing methods for genotyping of Mycobacterium tuberculosis complex strains 179 sources of Hubei and Hunan Province, and the combination of patient information and the resistant strains preliminary study This area is the popular features of tuberculosis. 179 MIRU data is given 24 points through the amplified DNA product of 24 sites, and analysis by agarose gel electrophoresis, MIRU genotyping methods to reach the level of strain identification; through cluster analysis is divided into 179 Mycobacterium tuberculosis four genotypes: A genotype 1.67%, genotype B 13.96% 59.77% C genotype D genotype 24.58%; C genotype proportion may Hubei and Hunan popular genotype. The geographical distribution of strains resistant and patient gender, the early retreatment state and geographical distribution analysis found that the C genotype strains with other genotype differences, that is, C genotype strains predominant strains in Hubei Province, to further analyze cause The possible reasons for this difference, and strain resistance and patient gender, the early retreatment state no correlation, its specific reasons need to be further studied.

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