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Study of the Communistic Characteristics of Vector Mosquito and the Prevalent Situation of Japanese Encephalitis in Yuanjiang-Red River, Yunnan Province

Author: GongDaoFang
Tutor: ZhouHongNing
School: Dali University
Course: Pathogen Biology
Keywords: Yuanjiang- Red River Japanese encephalitis Mosquitoes community serum antibody isolation and identification
CLC: R384
Type: Master's thesis
Year: 2010
Downloads: 11
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Abstract


Objectives:Through investigating Communistic Characteristics of Vector Mosquitoes and the prevalent situation of Japanese Encephalitis(JE) in Yuanjiang -Red River, Yunnan Province, to discuss the diversity characteristics of vector mosquitoes and the actual prevalent levels of JE.Contents:(1) Investigation the communistic characteristic of Vector mosquitoes in Yuanjian- Red River:Collecting mosquitoes in different environment gradients (longitude, latitude and altitude) of the Yuanjiang-Red River by lamp-traps in the whole night, in order to observe the compositions and distributional characteristics of JE vector mosquitoes. (2) The encephalitis serological investigation of Yuanjiang-Red River:Collected serum from various observation sits in healthy residents, using HI test to detect JE IgG. (3)The survey the natural carriers of the JEV on mosquito in the Yuanjiang-Red River:grinding the mosquitoes collected from field sites, RT-PCR and immunofluorescence test and other methods were used to identify the JE virus, the supernatant were inoculated C6/36 cells to isolate the JE virus.Methods:(1) investigation of the community characteristic of JE vector mosquitoes in the Yuanjiang-Red River:In the areas of north latitudes (23°～26°), east longitudes(100°～104°), and elevation (170m～1642m), By taking a census of focus combined with sampling survey in different latitude or longitude (every latitude or longitude as a horizontal gradient), different attitudes (every 500 meter height as a vertical gradient), different climates or covered by different plant, to research the diversity of mosquito vectors species and other biological properties in different habitats, using the lamp-traps to catch mosquitoes in human rooms and stables from different environmental gradients, so that observe their compositions and distributions of mosquitoes; analyzing datum of mosquito from different environmental gradients, and based on the specimens, information and data of mosquito attained in different environments to study their diversity. (2) Investigation of serum of JEV in the Yuanjiang-Red River:collecting healthy resident serum from Jinping Xinping Jingdu and Jinggu, and using the HI test to detect JE antibody IgG. (3) Investigation of JEV natural carriers from mosquitoes:Mosquitoes were captured in the field sites and classified, counted, registered, freeze them in liquid Nitrogen, and taken back to the laboratory; in Lab, grinding these mosquitoes, identifying JE virus by RT-PCR and immunofluorescence test and other methods, inoculating and isolating them by C6/36 cells.Results:(1) Total of 87875 indivual mosquitoes from 37 species(subspecies) in 6 genea were captured in the Yuanjiang-Red River, and Culex tritaeniorhynchus and Anopheles sinensis were the dominant species. The result analysis shows that the study area of mosquito species diversity and evenness is of significant positive correlation(r=0.951),and the ecological dominance into a high negative correlation (r=-0.976), negatively correlated with latitude (r=-0.561), and longitude gradient was positively correlated (=0.411),there is no significant regularity (r=0.177) with the elevation gradient, and species diversity (2) 803 healthy resident serum were collected from Jinping Xinping Jingdu and Jinggu, the positive rate was 61.89%, in which the JE antibody positive rate of Jingping County was higher than the others, and that of 1-5 year-old group was lower than the others. (3) Using two primer pairs of the JEV carry out RT-PCR for two kinds of 40 batch 3321 female adult mosquitoes, the result of detection is 4 strains for JE positive, which two strains were detected from Culex tritaeniorhynchus and the other 2 from An. sinensis, and these 4 JE viruses produced the significant cytopathic virus on the C6/36 cells, and confirmed the JEV by indirect immunofluorescence assay.Conclusions:(1) Culex tritaeniorhynchus and Anopheles sinensis were the dominant species of mosquito in the settlements region of Yuanjiang -Red River, mosquito diversities have some links with latitude gradient, the evenness of mosquitoes and ecological dominance. (2) The JE antibody levels in the local healthy residents are higher, as well as its prevalent degrees. (3). Japanese encephalitis vectors were complexity, and Culex tritaeniorhynchus and Anopheles sinensis belonged to the local JEV important vectors.

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