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Research on the Relationship between ABCG2 and Cells Arsenic-resesistance

Author: WangYi
Tutor: MuXiaoLing
School: Shihezi University
Course: Human Anatomy and Embryology
Keywords: ABCG2 Gene Expression Multidrug resistance Anti - arsenic resistance
CLC: R346
Type: Master's thesis
Year: 2009
Downloads: 5
Quote: 0
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Abstract


Objective: To detect over- expression of ABCG2 cell model arsenic tolerance change , the initial to investigate ABCG2 arsenic resistance generated relationship . Methods : ( 1) immunofluorescence chemical methods to detect ChAsE-BMSCs, arsenic resistant cells of bladder cancer and 23 patients with acute leukemia bone marrow ABCG2 expression ; ( 2 ) eukaryotic expression vector pEGFP-C1-ABCG2 converted , screening and sequencing identification , extraction , and access to the high purity of the recombinant plasmid , was transfected into 293T cells using liposome-mediated transfection method and Western- blot identification transfection results (3) trypan blue counting method to detect ABCG2 overexpression in 293T cells proliferation of impact (4) cytotoxicity assay ABCG2 over - expressing cells arsenicals tolerance change . Results: ( 1) immunofluorescence method detected two arsenic resistance cells ABCG2 protein expression were lower than the control group cells ; ( 2) immunofluorescence method detected 23 cases of acute leukemia patients ABCG2 protein expression intensity from low to high , as follows : control group , the onset and recurrent groups , the differences between the three was statistically significant ( P lt; 0.0001 ) ( 3 ) laser scanning confocal microscopy and Western-blot results prove successful transfection ; (4) ABCG2 overexpression reduced the proliferative capacity of cells ; (5) ABCG2 overexpression cells to arsenicals tolerance decreased . Conclusion: ( 1) ABCG2 protein arsenic resistant cells downregulated cells resistant to arsenic and ABCG2 expression was negatively correlated ; ( 2 ) of ABCG2 high expression is an unfavorable prognostic factor in patients with acute leukemia ; (3) successful establishment of overexpression of ABCG2 gene cell model;

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CLC: > Medicine, health > Basic Medical > Human biochemistry, molecular biology
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