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Morphology, Pacemaker Function of Interstitial Cells Cajal-like Cells in the Guinea Pig Urinary Bladder

Author: ZhuGuoDong
Tutor: WangQinZhang
School: Shihezi University
Course: Surgery
Keywords: Cajal -like cells Ultrastructure Calcium oscillations Calcium current Pacemaker cells
CLC: R334
Type: Master's thesis
Year: 2009
Downloads: 9
Quote: 0
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Abstract


Objective: The normal adult guinea pig bladder Cajal -like interstitial cells of this subject as an object of study , bladder Cajal - like interstitial cells (interstitial cells of Cajal, ICCs) ultrastructure and electrophysiological properties , explore whether it is the bladder pacemaker cells , to provide a new entry point for the clinical treatment of certain diseases ( such as detrusor instability, DI) . Methods : In this study, the use of enzyme digestion , ICCs -like cells under in vitro culture conditions , transmission electron microscopy to observe the ultrastructure of the normal adult guinea pig bladder ICCs -like cells ; the Cajal kind using laser scanning confocal microscope imaging techniques the record Fluo4AM load between the change in fluorescence intensity of the calcium ions in the stromal cells , the fluorescence intensity is representative of the change of the intracellular calcium ion concentration . ; Observe whether a calcium current in order to verify whether the bladder pacemaker cells using patch-clamp technique in the whole-cell recording mode . Results: TEM observations CCs like cytoplasmic mitochondria , abundant rough endoplasmic reticulum , smooth endoplasmic reticulum scattered , the intermediate filament difficult to see , no microtubules ; continuous use of laser confocal microscopy imaging technology scan, see vitro cultured primary bladder Cajal -like interstitial cells of two types of calcium oscillations : a class of amplitude , frequency slow regular calcium oscillations ; another type of small amplitude , frequency of fast and irregular , and the formation of a network of bladder Cajal - like interstitial cells calcium shock amplitude significantly higher than single bladder Cajal -like interstitial cells . Detrusor cell calcium oscillations of small amplitude irregular amplitude was significantly lower than the interstitial cells of Cajal -like first class calcium oscillations amplitude ; patch-clamp the test bladder Cajal -like cells calcium ion current is not obvious . Conclusion : electron microscopy to Cajal -like cells have unique ultrastructure the ultrastructure of ICCs in the gastrointestinal similar ; bladder Cajal - like cells with spontaneous and regular calcium oscillations similar gastrointestinal pacemaker cells of Cajal interstitial cells (interstitial cells of Cajal, ICCs) spontaneous calcium oscillations detrusor no spontaneous calcium shock . Tip: part of the bladder Cajal - like cells are the pacemaker cells of the bladder contractile activity ; another part of the bladder Cajal -like cells may play a conduction coupling of the role of information .

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